Mutations in a Leucine-Rich Repeat Receptor-Like Kinase gene result in male sterility and reduction in the number and size of fruit warts in cucumber (Cucumis sativus L.)

被引:2
作者
Zhang, Haiqiang [1 ]
Luo, Yanjie [1 ]
Zhen, Wenlong [1 ]
Li, Xin [1 ]
Liu, Mengying [1 ]
Liu, Peng [1 ]
Zhang, Gaoyuan [2 ]
Chen, Peng [3 ]
Weng, Yiqun [4 ]
Yue, Hongzhong [5 ]
Li, Yuhong [1 ]
机构
[1] Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China
[2] Gansu Agr Univ, Coll Hort, Lanzhou 730070, Gansu, Peoples R China
[3] Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China
[4] Univ Wisconsin, Vegetable Crops Res Unit, USDA ARS, Madison, WI 53706 USA
[5] Gansu Acad Agr Sci, Vegetable Res Inst, Lanzhou 730070, Gansu, Peoples R China
基金
中国国家自然科学基金;
关键词
TRICHOME DEVELOPMENT; SPINE DENSITY; IDENTIFICATION; PROTEIN; INHERITANCE; INITIATION; PHENOTYPE; ENCODES; FLOWER; MUTANT;
D O I
10.1007/s00122-024-04790-y
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Key messageMutations in the CsEMS1 gene result in male sterility and reduced wart number and density.AbstractMale sterility and fruit wart formation are two significant agronomic characteristics in cucumber (Cucumis sativus), yet knowledge of our underlying genetics is limited. In this study, we identified an EMS-induced male sterility and few small warts mutant (msfsw). Histological observations revealed defects the absence of tapetum, meiotic aberration and impaired microspore formation in the anthers of the mutant. The mutant also exhibits a reduction in both the size and number of fruit spines and fruit tubercules. Genetic analysis revealed that a single recessive gene is responsible for the mutant phenotypes. BSA-Seq and fine genetic mapping mapped the msfsw locus to a 63.7 kb region with four predicted genes. Multiple lines of evidence support CsEMS1(CsaV3_3G016940) as the candidate for the mutant allele which encodes an LRR receptor-like kinase, and a non-synonymous SNP inside the exon of CsEMS1 is the causal polymorphisms for the mutant phenotypes. This function of CsEMS1 in determination of pollen fertility was confirmed with generation and characterization of multiple knockout mutations with CRISPR/Cas9 based gene editing. In the wild-type (WT) plants, CsEMS1 was highly expressed in male flowers. In the mutant, the expression level of CsEMS1, several tapetum identity-related genes, and trichome-related genes were all significantly reduced as compared with the wild-type. Protein-protein interaction assays revealed physical interactions between CsEMS1 and CsTPD1. Quantitation of endogenous phytohormones revealed a reduction in the ethylene precursor ACC in CsEMS1 knockout lines. This work identified an important role of CsEMS1 in anther and pollen development as well as fruit spine/wart development in cucumber.
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页数:14
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共 63 条
[1]   Ethylene plays a dual role in sex determination and fruit shape in cucurbits [J].
Boualem, Adnane ;
Berthet, Serge ;
Devani, Ravi Sureshbhai ;
Camps, Celine ;
Fleurier, Sebastien ;
Morin, Halima ;
Troadec, Christelle ;
Giovinazzo, Nathalie ;
Sari, Nebahat ;
Dogimont, Catherine ;
Bendahmane, Abdelhafid .
CURRENT BIOLOGY, 2022, 32 (11) :2390-+
[2]   The HD-ZIP IV transcription factor GL2-LIKE regulates male flowering time and fertility in cucumber [J].
Cai, Yanling ;
Bartholomew, Ezra S. ;
Dong, Mingming ;
Zhai, Xuling ;
Yin, Shuai ;
Zhang, Yaqi ;
Feng, Zhongxuan ;
Wu, Licai ;
Liu, Wan ;
Shan, Nan ;
Zhang, Xiao ;
Ren, Huazhong ;
Liu, Xingwang .
JOURNAL OF EXPERIMENTAL BOTANY, 2020, 71 (18) :5425-5437
[3]   EXS, a putative LRR receptor kinase, regulates male germline cell number and tapetal identity and promotes seed development in Arabidopsis [J].
Canales, C ;
Bhatt, AM ;
Scott, R ;
Dickinson, H .
CURRENT BIOLOGY, 2002, 12 (20) :1718-1727
[4]   Molecular basis of cucumber fruit domestication [J].
Che, Gen ;
Zhang, Xiaolan .
CURRENT OPINION IN PLANT BIOLOGY, 2019, 47 :38-46
[5]   The WD-Repeat Protein CsTTG1 Regulates Fruit Wart Formation through Interaction with the Homeodomain-Leucine Zipper I Protein Mict [J].
Chen, Chunhua ;
Yin, Shuai ;
Liu, Xingwang ;
Liu, Bin ;
Yang, Sen ;
Xue, Shudan ;
Cai, Yanling ;
Black, Kezia ;
Liu, Huiling ;
Dong, Mingming ;
Zhang, Yaqi ;
Zhao, Binyu ;
Ren, Huazhong .
PLANT PHYSIOLOGY, 2016, 171 (02) :1156-1168
[6]   Transcriptome profiling reveals roles of meristem regulators and polarity genes during fruit trichome development in cucumber (Cucumis sativus L.) [J].
Chen, Chunhua ;
Liu, Meiling ;
Jiang, Li ;
Liu, Xiaofeng ;
Zhao, Jianyu ;
Yan, Shuangshuang ;
Yang, Sen ;
Ren, Huazhong ;
Liu, Renyi ;
Zhang, Xiaolan .
JOURNAL OF EXPERIMENTAL BOTANY, 2014, 65 (17) :4943-4958
[7]   BES1 is activated by EMS1-TPD1-SERK1/2-mediated signaling to control tapetum development in Arabidopsis thaliana [J].
Chen, Weiyue ;
Lv, Minghui ;
Wang, Yanze ;
Wang, Ping-An ;
Cui, Yanwei ;
Li, Meizhen ;
Wang, Ruoshi ;
Gou, Xiaoping ;
Li, Jia .
NATURE COMMUNICATIONS, 2019, 10 (1)
[8]   Down-Regulating CsHT1, a Cucumber Pollen-Specific Hexose Transporter, Inhibits Pollen Germination, Tube Growth, and Seed Development [J].
Cheng, Jintao ;
Wang, Zhenyu ;
Yao, Fengzhen ;
Gao, Lihong ;
Ma, Si ;
Sui, Xiaolei ;
Zhang, Zhenxian .
PLANT PHYSIOLOGY, 2015, 168 (02) :635-+
[9]   Genic male and female sterility in vegetable crops [J].
Cheng, Zhihua ;
Song, Weiyuan ;
Zhang, Xiaolan .
HORTICULTURE RESEARCH, 2023, 10 (01)
[10]   A New Glabrous Gene (csgl3) Identified in Trichome Development in Cucumber (Cucumis sativus L.) [J].
Cui, Jin-Ying ;
Miao, Han ;
Ding, Li-Hong ;
Wehner, Todd C. ;
Liu, Pan-Na ;
Wang, Ye ;
Zhang, Sheng-Ping ;
Gu, Xing-Fang .
PLOS ONE, 2016, 11 (02)