A WUSCHEL-related homeobox transcription factor, SlWOX4, negatively regulates drought tolerance in tomato

被引：3

作者：

Li, Hui ^{[1
]}

Ma, Wanying ^{[1
]}

Wang, Xiao ^{[1
]}

Hu, Hongling ^{[1
]}

Cao, Lina ^{[1
]}

Ma, Hui ^{[1
]}

Lin, Jingwei ^{[1
]}

Zhong, Ming ^{[1
]}

机构：

[1] Shenyang Agr Univ, Coll Biosci & Biotechnol, Key Lab Agr Biotechnol Liaoning Prov, Shenyang, Peoples R China

来源：

PLANT CELL REPORTS | 2024年 / 43卷 / 11期

关键词：

Tomato; SlWOX4 transcription factor; Drought stress; Reactive oxygen species; Abscisic acid; ABSCISIC-ACID BIOSYNTHESIS; 2C PROTEIN PHOSPHATASES; KEY ENZYME; STRESS; GENE; SALT; GROWTH; ABA; 8'-HYDROXYLASE; KINASES;

D O I：

10.1007/s00299-024-03333-5

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Key messageCRISPR/Cas9-mediated knockout of SlWOX4 gene in tomato enhances tolerance to drought stress.AbstractDrought stress is one of the major abiotic factors that seriously affects plant growth and crop yield. WUSCHEL-related homeobox (WOX) transcription factors are involved in plant growth, development and stress response. However, little is known about the role of WOX genes in drought tolerance in tomato. Here, SlWOX4, a member of the WOX family in tomato, was functionally characterized in mediating drought tolerance. SlWOX4 was homologous to Nicotiana tabacum NtWOX4 with a conserved HD domain, and was localized in the nucleus. SlWOX4 was significantly down-regulated by drought and abscisic acid (ABA) treatments. The loss-of-function mutations of SlWOX4 produced using the CRISPR-Cas9 system in tomato improved drought tolerance by reducing water loss rate and enhancing stomatal closure. In addition, the wox4 lines exhibited reduced accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), increased antioxidant enzyme activity, proline contents and ABA contents under drought stress. Moreover, gene editing of SlWOX4 in tomato enhanced drought tolerance by regulating the expression of genes encoding antioxidants and ABA signaling molecules. In summary, SlWOX4 gene might negatively regulate drought stress tolerance in tomato and has great potential as a drought-resistant crop-breeding target genes.

引用

页数：13

共 82 条

[1] SlWRKY81 reduces drought tolerance by attenuating proline biosynthesis in tomato [J].