Gut microbiota-mediated bile acid transformations regulate the transport of aflatoxin B1 from the intestine to the liver in piglets

被引:0
作者
Mao, Jiangdi [1 ]
Wei, Yusen [1 ]
Ni, Zhixiang [1 ]
Zhang, Jinzhi [1 ]
Zhu, Junli [2 ]
Wang, Haifeng [1 ]
机构
[1] Zhejiang Univ, Coll Anim Sci, Key Lab Mol Anim Nutr, Minist Educ, Hangzhou 310000, Peoples R China
[2] Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Food Safety Key Lab Zhejiang Prov, Hangzhou 310018, Peoples R China
关键词
Aflatoxin; Bile acid; FXR; Microbiota; Piglet; FEEDBACK-REGULATION; METABOLISM; DEOXYNIVALENOL; PERFORMANCE; OCHRATOXIN; ACTIVATION; STRAINS; FXR; B-1;
D O I
10.1186/s40104-025-01169-x
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
BackgroundAflatoxins have been reported as a significant pollutant in feed, capable of causing harm to the liver, gastrointestinal tract and kidneys of piglets. However, research on the interactions among aflatoxin B1 (AFB1), bile acid (BA) metabolism and gut microbiota is limited.MethodsIn this study, piglets were treated with AFB1 and antibiotics (ABX) to evaluate the interaction between AFB1 and gut microbiota. Subsequently, the roles of the farnesoid X receptor (FXR) and sterol 12 alpha-hydroxylase (CYP8B1) in AFB1 absorption were studied by using FXR agonists obeticholic acid (OCA) and Cyp8b1-knockout (KO) mice, respectively.ResultAFB1 inhibited bile salt hydrolase (BSH) activity in ileal microbiota, downregulated ileal FXR expression, and upregulated CYP8B1 expression in liver, increasing the proportion of 12 alpha-OH BAs and potentially enhancing AFB1 absorption. ABX treatment reduced AFB1 absorption and liver damage, and unexpectedly increased BSH activity, counteracting the AFB1-induced downregulation of FXR and upregulation of CYP8B1. OCA reactivated ileal FXR, reduced AFB1 absorption, and alleviated liver damage. Furthermore, Cyp8b1-KO mice showed increased resistance to AFB1-induced liver damage by lowering AFB1 absorption.ConclusionsThese results underscore the significance of gut microbiota and BAs in AFB1 absorption, suggesting new strategies to mitigate health risks from AFB1 in piglets.
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页数:18
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