Diagnostic Value of Targeted Next-generation Sequencing in Pulmonary Mycobacterial Infections

被引:0
作者
Tao, Yu [1 ]
Zhou, Zi-wei [1 ]
Duan, Yi-fei [1 ]
Wang, Jian-miao [1 ]
机构
[1] Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Resp & Crit Care Med, Wuhan 430030, Peoples R China
来源
CURRENT MEDICAL SCIENCE | 2024年
关键词
targeted next-generation sequencing; Mycobacterium tuberculosis; nontuberculous mycobacteria; bronchoalveolar lavage fluid; BRONCHOALVEOLAR LAVAGE; DRUG-RESISTANCE; GENE XPERT; TUBERCULOSIS; SPUTUM; MICROSCOPY;
D O I
10.1007/s11596-024-2937-4
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
ObjectiveThis study aimed to explore the diagnostic value of novel technique-targeted next-generation sequencing (tNGS) of bronchoalveolar lavage fluid (BALF) in pulmonary mycobacterial infections. MethodsThis retrospective study was conducted on patients who underwent bronchoscopy and tNGS, smear microscopy, and mycobacterial culture of BALF. Patients with positive Mycobacterium tuberculosis (MTB) culture or GeneXpert results were classified into the tuberculosis case group. Those diagnosed with nontuberculous mycobacteria (NTM)-pulmonary disease (NTM-PD) composed the case group of NTM-PD patients. The control group comprised patients without tuberculosis or NTM-PD. Sensitivity, specificity, and receiver operating characteristic (ROC) curves were used to evaluate the diagnostic performance. ResultsFor tuberculosis patients with positive mycobacterial culture results, the areas under the ROC curves (AUCs) for tNGS, GeneXpert, and smear microscopy were 0.975 (95% CI: 0.935, 1.000), 0.925 (95% CI: 0.859, 0.991), and 0.675 (95% CI: 0.563, 0.787), respectively. For tuberculosis patients with positive GeneXpert results, the AUCs of tNGS, culture, and smear microscopy were 0.970 (95% CI: 0.931, 1.000), 0.850 (95% CI: 0.770, 0.930), and 0.680 (95% CI: 0.579, 0.781), respectively. For NTM-PD, the AUCs of tNGS, culture, and smear-positive but GeneXpert-negative results were 0.987 (95% CI: 0.967, 1.000), 0.750 (95% CI: 0.622, 0.878), and 0.615 (95% CI: 0.479, 0.752), respectively. The sensitivity and specificity of tNGS in NTM-PD patients were 100% and 97.5%, respectively. ConclusiontNGS demonstrated superior diagnostic efficacy in mycobacterial infection, indicating its potential for clinical application.
引用
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页码:947 / 953
页数:7
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