TCTEX1D2 is essential for sperm flagellum formation in mice

被引：2

作者：

Harima, Ryua ^{[1
]}

Hara, Kenshiro ^{[1
]}

Tanemura, Kentaro ^{[1
,2
]}

机构：

[1] Tohoku Univ, Grad Sch Agr Sci, Lab Anim Reprod & Dev, 468-1 Aramaki Aoba,Aoba Ku, Sendai, Miyagi 9808572, Japan

[2] Tohoku Univ, Grad Sch Agr Sci, Lab Reprod Technol Repro SOLEIL, 468-1 Aramaki Aoba,Aoba Ku, Sendai, Miyagi 9808572, Japan

来源：

SCIENTIFIC REPORTS | 2025年 / 15卷 / 01期

基金：

日本科学技术振兴机构;

关键词：

Spermatogenesis; Sperm flagella; Cytoplasmic dynein 2; Inner dynein arm; Male infertility; CILIARY PROTEIN TRAFFICKING; INTRAFLAGELLAR TRANSPORT; CYTOPLASMIC DYNEIN-2; SENSORY CILIA; LIGHT-CHAIN; INNER; IFT; DROSOPHILA; PARTICLES; DEFECTS;

D O I：

10.1038/s41598-024-83424-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Flagella and cilia are widely conserved motile structures, in mammalian, sperm possess flagella. Large protein complexes called dynein, including cytoplasmic dynein 2 and axonemal dynein, play a role in the formation of cilia and flagella. The function of each subunit component of dynein complexes in sperm flagellum formation remains unclear. One such subunit is TCTEX1D2. Co-immunoprecipitation studies showed that TCTEX1D2 interacted with cytoplasmic dynein 2 subunits WDR34, WDR60, and DYNLT1 in the testes. Furthermore, TCTEX1D2 also interacted with WDR63 and WDR78, subunits of inner dynein arm, which is axonemal dynein. Tctex1d2-/- mice generated in this study exhibited male infertility due to flagellar dysplasia, and the axonemal structures were disrupted inside the flagella. Further, the localization of cytoplasmic dynein 2 subunits was abnormal in in Tctex1d2-/- mice. In contrast, the motile cilia of Tctex1d2-/- mice were normal. Overall, we revealed that TCTEX1D2 is important for the assembly of cytoplasmic dynein 2 and inner dynein arm and functions in two distinct dynein complexes during mouse sperm flagellum formation. This is only in sperm flagellum formation, not in cilia formation.

引用

页数：15

共 55 条

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