Vector competence of Culex quinquefasciatus from Santiago Island, Cape Verde, to West Nile Virus: exploring the potential effect of the vector native Wolbachia

被引：0
作者：
da Moura, Aires Januario Fernandes [1 ,2 ]
Tomaz, Filipe [1 ]
Melo, Tiago [1 ]
Seixas, Goncalo [1 ]
Sousa, Carla A. [1 ]
Pinto, Joao [1 ]
机构：
[1] Univ Nova Lisboa UNL, Associate Lab Translat & Innovat Global Hlth LA RE, Global Hlth & Trop Med GHTM, Inst Higiene & Med Trop IHMT, Rua Junqueira 100, P-1349008 Lisbon, Portugal
[2] Univ Jean Piaget Cabo Verde, Unidade Ciencias Nat Vida & Ambiente, Praia, Cape Verde
来源：
PARASITES & VECTORS | 2024年 / 17卷 / 01期
关键词：
<italic>Culex</italic><italic>quinquefasciatus</italic>; West Nile virus; <italic>Wolbachia</italic>; Vector competence; Cape Verde; DIPTERA-CULICIDAE; INCLUDING RECORDS; PIPIENS COMPLEX; 9; TAXA; MOSQUITOS; INFECTION; SYMBIONT; LINEAGE; DENGUE; BIRDS;
D O I：
10.1186/s13071-024-06609-7
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
BackgroundCulex quinquefasciatus plays a crucial role as a vector of West Nile virus (WNV). This mosquito species is widely distributed in Cape Verde, being found in all inhabited islands of the archipelago. However, no data are currently available on the susceptibility of the local mosquito population to WNV. This study aimed to assess the vector competence of Cx. quinquefasciatus mosquitoes from Santiago Island, Cape Verde, for WNV and to explore the potential impact of its native Wolbachia on virus transmission.MethodsWolbachia-infected and uninfected Cx. quinquefasciatus female mosquitoes were exposed to WNV lineage 1 PT6.39 strain using a Hemotek membrane feeding system. Mosquito samples, including the body, legs, wings and saliva, were collected at days 7, 14 and 21 post-infection (dpi) to assess WNV infection through one-step quantitative real-time PCR (RT-qPCR).ResultsCulex quinquefasciatus from Cape Verde exhibited high susceptibility to the tested strain of WNV. Also, treated females without their native Wolbachia exhibited significantly higher WNV load in their bodies and greater dissemination rate at 7 dpi than their wild-type counterparts carrying Wolbachia.ConclusionsThe high susceptibility to WNV of Cx. quinquefasciatus from Cape Verde poses a potential risk for virus transmission in the archipelago. However, Wolbachia infection in this mosquito species seems to confer protection against WNV dissemination in the early stages of viral infection. Additional research is required to uncover the mechanisms driving this protection and its potential impact on WNV transmission.
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页数：12
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  • [1] Karim S.U., Bai F., Introduction to West Nile virus, Methods Mol Biol, 2585, pp. 1-7, (2023)
  • [2] Clark M.B., Schaefer T.J., West Nile Virus, (2024)
  • [3] Mencattelli G., Ndione M.H.D., Rosa R., Marini G., Diagne C.T., Diagne M.M., Et al., Epidemiology of West Nile virus in Africa: an underestimated threat, PLoS Negl Trop Dis, 16, (2022)
  • [4] Ulbert S., West Nile virus vaccines—current situation and future directions, Hum Vaccin Immunother, 15, pp. 2337-2342, (2019)
  • [5] Fiacre L., Pages N., Albina E., Richardson J., Lecollinet S., Gonzalez G., Molecular determinants of West Nile virus virulence and pathogenesis in vertebrate and invertebrate hosts, Int J Mol Sci, 21, (2020)
  • [6] Garrigos M., Garrido M., Panisse G., Veiga J., Martinez-de la Puente J., Interactions between West Nile virus and the microbiota of Culex pipiens vectors: a literature review, Pathogens, 12, (2023)
  • [7] Koenraadt C.J.M., Mohlmann T.W.R., Verhulst N.O., Spitzen J., Vogels C.B.F., Effect of overwintering on survival and vector competence of the West Nile virus vector Culex pipiens, Parasit Vectors, 12, (2019)
  • [8] Romo H., Papa A., Kading R., Clark R., Delorey M., Brault A.C., Comparative vector competence of north American Culex pipiens and Culex quinquefasciatus for African and European lineage 2 West Nile viruses, Am J Trop Med Hyg, 98, pp. 1863-1869, (2018)
  • [9] Fortuna C., Remoli M.E., Di Luca M., Severini F., Toma L., Benedetti E., Et al., Experimental studies on comparison of the vector competence of four Italian Culex pipiens populations for West Nile virus, Parasit Vectors, 8, (2015)
  • [10] Tokash-Peters A.G., Jabon J.D., Fung M.E., Peters J.A., Lopez S.G., Woodhams D.C., Trans-generational symbiont transmission reduced at high temperatures in a West Nile virus vector mosquito Culex quinquefasciatus, Front Trop Dis, 3, pp. 1-12, (2022)
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