Emerging insights into intravital imaging, unraveling its role in cancer immunotherapy

被引：0

作者：

Yang, Minfeng ^{[1
]}

Hou, Shiqiang ^{[3
]}

Chen, Yao ^{[2
]}

Chen, Hongzhao ^{[4
]}

Chu, Minjie ^{[1
]}

Liu, Song-Bai ^{[2
]}

机构：

[1] Nantong Univ, Sch Publ Hlth, Nantong, Peoples R China

[2] Suzhou Vocat Hlth Coll, Jiangsu Prov Engn Res Ctr Mol Target Therapy & Com, Suzhou 215009, Peoples R China

[3] Anhui Med Univ, Affiliated Chuzhou Hosp, Peoples Hosp Chuzhou 1, Chuzhou, Peoples R China

[4] Hong Kong Polytech Univ, Dept Hlth Technol & Informat, Kowloon, Hong Kong, Peoples R China

来源：

CANCER IMMUNOLOGY IMMUNOTHERAPY | 2025年 / 74卷 / 03期

关键词：

Intravital imaging; Immunotherapy; Tumor angiogenesis; Tumor immunity; Imaging windows; TUMOR-ASSOCIATED MACROPHAGES; T-CELL MOTILITY; DENDRITIC CELLS; ANTITUMOR IMMUNITY; MOUSE MODEL; VISUALIZATION; RESPONSES; DYNAMICS; MICROSCOPY; ANTIBODIES;

D O I：

10.1007/s00262-025-03944-1

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Cancer immunotherapy has attracted great attention as a potential therapeutic approach for advanced malignancies due to its promising survival benefits. Comprehension of intricate interactions between the tumor microenvironment (TME) and immune checkpoint inhibitors (ICIs) is crucial for optimizing and improving immunotherapies. Currently, several experimental strategies are available to monitor this complexity but most of them fail to facilitate real-time monitoring of the immune response such as cellular phagocytosis and cytolysis. Consequently, the application of intravital imaging has been extensively studied in the domain of cancer immunotherapy. Intravital imaging has been proven to be a powerful real-time imaging modality that provides insights into intratumoral immune responses, cellular metabolic signatures, tumor vasculature, and cellular functions. This review aims to provide a comprehensive overview of the latest research on intravital imaging in cancer immunotherapy, especially addressing how intravital imaging sheds light on essential features of tumor immunity, immune infiltrations, tumor angiogenesis, and aids in the clarification of underlying immunotherapeutic mechanisms. Moreover, a variety of labeling tools, imaging windows and models for real-time visualizations of TME are also summarized. We will also investigate the full potential of using intravital imaging to circumvent the limitations of currently available imaging modalities, which hold promise to advent efficient immunotherapy for cancer patients.

引用

页数：18