Revealing real-time 3D in vivo pathogen dynamics in plants by label-free optical coherence tomography

被引：5
作者：
de Wit, Jos [1 ]
Tonn, Sebastian [2 ]
Shao, Mon-Ray [2 ]
van den Ackerveken, Guido [2 ]
Kalkman, Jeroen [1 ]
机构：
[1] Delft Univ Technol, Dept Imaging Phys, Delft, Netherlands
[2] Univ Utrecht, Dept Biol, Translat Plant Biol, Utrecht, Netherlands
来源：
NATURE COMMUNICATIONS | 2024年 / 15卷 / 01期
基金：
荷兰研究理事会;
关键词：
GREEN FLUORESCENT PROTEIN; BREMIA-LACTUCAE; QUANTIFICATION; MICROSCOPY; CONTRAST; GROWTH; GFP;
D O I：
10.1038/s41467-024-52594-x
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Microscopic imaging for studying plant-pathogen interactions is limited by its reliance on invasive histological techniques, like clearing and staining, or, for in vivo imaging, on complicated generation of transgenic pathogens. We present real-time 3D in vivo visualization of pathogen dynamics with label-free optical coherence tomography. Based on intrinsic signal fluctuations as tissue contrast we image filamentous pathogens and a nematode in vivo in 3D in plant tissue. We analyze 3D images of lettuce downy mildew infection (Bremia lactucae) to obtain hyphal volume and length in three different lettuce genotypes with different resistance levels showing the ability for precise (micro) phenotyping and quantification of the infection level. In addition, we demonstrate in vivo longitudinal imaging of the growth of individual pathogen (sub)structures with functional contrast on the pathogen micro-activity revealing pathogen vitality thereby opening a window on the underlying molecular processes.
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页数：9
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