Production of secondary metabolites in callus cultures of Scutellaria baicalensis L. and assessment of their anti-inflammatory and antioxidant efficacy in ulcerative colitis rats

Baikal skullcap or Chinese (Scutellaria baicalensis L.) is an interesting plant with promising medicinal properties; however, traditional cultivation methods are time-consuming, and yield variations can be significant; callus culture is considered one of the solutions to overcome these limitations because the callus culture provides an effective, alternative for the consistent production of secondary metabolites. For callus production of S. baicalensis L., the in vitro germinating seedlings were cultured on MS medium containing 1.0 mg/L 6-benzyladenine (BAP) and 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). Three culture lines were established, and the best growth index represented in fresh and dry weight was obtained from line No. 1. S. baicalensis L. callus extract was performed on the best callus line in the stationary phase for in vitro assays. The chemical analysis, antioxidant tests, proline, flavonoids, phenolics, and macronutrient content were assessed. Therefore, this paper aims to evaluate the effectiveness of secondary metabolites in S. baicalensis L. callus and to study its biological effect on recurrent ulcerative colitis (UC). Conventional treatment of UC has focused on suppressing immunological responses instead of addressing which are (UC) underlying causes. Recurrent UC is caused by oxidative stress and inflammation that lead to chronic inflammation of the inner lining of the colon and rectum. According to the findings, secondary metabolites in S. baicalensis L. callus cultures increased antioxidant activity. This improvement in oxidative activity was positively correlated with the potential to reduce UC in vivo.