Identification of allelochemicals under continuous cropping of Morchella mushrooms

被引：0

作者：

Yin, Qi ^{[1
]}

Zhang, Wenchang ^{[1
]}

Shi, Hanfei ^{[2
]}

He, Peixin ^{[1
]}

Zhang, Fengming ^{[3
,4
]}

Zhang, Jin ^{[3
,4
]}

Li, Bin ^{[5
]}

Shi, Xiaofei ^{[3
,4
]}

Liu, Wei ^{[3
,4
]}

Yu, Fuqiang ^{[3
,4
]}

机构：

[1] Zhengzhou Univ Light Ind, Sch Food & Biol Engn, Zhengzhou 450002, Peoples R China

[2] Luanchuan Cty Financial & Technol Serv Ctr, Luanchuan 471500, Peoples R China

[3] Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Peoples R China

[4] Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Fungal Divers & Green Dev, Kunming 650201, Peoples R China

[5] Zhengzhou Inst Agr Sci & Technol, Zhengzhou 450005, Peoples R China

来源：

SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期

关键词：

Ascomycota; 4-Coumaric acid; GC-MS; Morel; Vanillic acid; OPLS-DA; BIOSYNTHESIS; METABOLISM; ISSUES;

D O I：

10.1038/s41598-024-82542-0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Continuous cropping obstacle has been becoming the bottleneck for the stable development of morel cultivation. The allelopathic effect of soil allelochemicals may play an instrumental role in the morel soil sickness. In this study, the allelochemicals were identified by gas chromatography-mass spectrometry (GC-MS) combined with in vitro bioassay. A total of 61 chemical substances were identified through the GC-MS analysis of 12 replanting and control soil samples, comprising 10 phenolic acids, 36 acids, 3 aldehydes, etc. Among which, 15 compounds with values of variable importance for the projection (VIP) greater than 1 in the orthogonal partial least squares-discriminant analysis (OPLS-DA) were selected as the differential metabolites between soil samples of continuous cropping and control. The bioassay showed that 4-coumaric acid and vanillic acid exhibited inhibitory effect on mycelial growth of three cultivated Morchella mushrooms (M. sextelata, M. eximia and M. importuna) under soil native concentrations. Analysis of potential biosynthetic pathways of phenolic acids found that the 3 cultivable Morchella mushrooms are unable to synthesize phenolic acid allelochemicals. Therefore, although they were detected in trace amounts in static culture broth of M. sextelata, the two phenolic acids can only be defined as morel allelochemicals rather than autotoxins. Taken together, we found two morel allelochemicals that may derive from morel related microbes and plants, which will be helpful for further fundamental study and application in morel artificial cultivation.

引用

页数：11

共 58 条

[1]

Du X.H., Zhao Q., Yang Z.L., A review on research advances, issues, and perspectives of morels, Mycology, 6, pp. 78-85, (2015)

[2]

Liu Q., Ma H., Zhang Y., Dong C., Artificial cultivation of true morels: Current state, issues and perspectives, Crit. Rev. Biotechnol, 38, pp. 259-271, (2018)

[3]

Sambyal K., Singh R.V., A comprehensive review on Morchella importuna: Cultivation aspects, phytochemistry, and other significant applications, Folia Microbiol, 66, pp. 147-157, (2021)

[4]

Tietel Z., Masaphy S., True morels (Morchella)—nutritional and phytochemical composition, health benefits and flavor: A review, Crit. Rev. Food Sci. Nutr, 58, pp. 1888-1901, (2017)

[5]

Liu W., Cai Y., Ma X., He P., Evaluation system of cultivating suitability for the productive strains of Morchella mushrooms, J. Light Ind, 37, 3, pp. 50-57, (2022)

[6]

Liu W., Et al., Large-scale field cultivation of Morchella and relevance of basic knowledge for its steady production, J. Fungi, 9, (2023)

[7]

Chen B., Et al., Dazomet changes microbial communities and improves morel mushroom yield under continuous cropping, Front. Microbiol, 14, (2023)

[8]

Ma Z., Et al., Obstacles in continuous cropping: Mechanisms and control measures, Adv. Agron, 179, pp. 205-256, (2023)

[9]

Yin Q., Et al., Allelopathic effects of phenolic acid extracts on Morchella mushrooms, pathogenic fungus, and soil–dominant fungus uncover the mechanism of morel continuous cropping obstacle, Arch. Microbiol, 206, (2024)

[10]

Liu W., Et al., Determining why continuous cropping reduces the production of the morel Morchella sextelata, Front. Microbiol, 13, (2022)

← 1 2 3 4 5 6 →