The chromatin remodeling factor OsINO80 promotes H3K27me3 and H3K9me2 deposition and maintains TE silencing in rice

被引：2

作者：

Du, Kangxi ^{[1
,2
]}

Wu, Jiabing ^{[1
]}

Wang, Jiachen ^{[1
]}

Xie, Wenhao ^{[1
]}

Yin, Liufan ^{[1
]}

Li, Xiang ^{[1
]}

Li, Chao ^{[1
]}

Dong, Aiwu ^{[1
]}

机构：

[1] Fudan Univ, Inst Plant Biol, Collaborat Innovat Ctr Genet & Dev, Sch Life Sci,State Key Lab Genet Engn,Dept Biochem, Shanghai, Peoples R China

[2] Sichuan Agr Univ, Rice Res Inst, State Key Lab Crop Gene Explorat & Utilizat Southw, Chengdu, Peoples R China

来源：

NATURE COMMUNICATIONS | 2024年 / 15卷 / 01期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

DNA METHYLATION; TRANSPOSABLE ELEMENTS; HISTONE METHYLATION; INO80; H2A.Z; COMPLEX; IDENTIFICATION; TRANSCRIPTION; VISUALIZATION; MUTATIONS;

D O I：

10.1038/s41467-024-55387-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The INO80 chromatin remodeling complex plays a critical role in shaping the dynamic chromatin environment. The diverse functions of the evolutionarily conserved INO80 complex have been widely reported. However, the role of INO80 in modulating the histone variant H2A.Z is controversial. Moreover, whether INO80 helps regulate heterochromatin remains unknown. Here, we characterize the regulatory effects of OsINO80 on protein-coding genes and transposable elements (TEs) in rice. Upon OsINO80 overexpression in rice, we found three types of OsINO80-occupied regions with different chromatin signatures: type I (enriched with H2A.Z), type II (enriched with H3K9me2), and type III (deficient in H2A.Z/H3K9me2). Loss of OsINO80 results in a decrease in H3K27me3, but not H2A.Z, at type I regions as well as a decrease in H3K9me2 at type II regions, which correlates with TE activation and transposition. Our findings reveal that OsINO80 facilitates H3K27me3 establishment, promotes H3K9me2 deposition, and maintains TE silencing.

引用

页数：11

共 76 条

[1] Removal of H2A.Z by INO80 promotes homologous recombination [J].

Alatwi, Hanan E. ;

Downs, Jessica A. .

EMBO REPORTS, 2015, 16 (08) :986-994

[2] Giant Transposons in Eukaryotes: Is Bigger Better? [J].

Arkhipova, Irina R. ;

Yushenova, Irina A. .

GENOME BIOLOGY AND EVOLUTION, 2019, 11 (03) :906-918

[3]

Bao Yunhe, 2007, Cell, V129, P632, DOI 10.1016/j.cell.2007.04.018

[4] The Contributions of Transposable Elements to the Structure, Function, and Evolution of Plant Genomes [J].

Bennetzen, Jeffrey L. ;

Wang, Hao .

ANNUAL REVIEW OF PLANT BIOLOGY, VOL 65, 2014, 65 :505-530

[5] Genome-Wide Association of Histone H3 Lysine Nine Methylation with CHG DNA Methylation in Arabidopsis thaliana [J].

Bernatavichute, Yana V. ;

Zhang, Xiaoyu ;

Cokus, Shawn ;

Pellegrini, Matteo ;

Jacobsen, Steven E. .

PLOS ONE, 2008, 3 (09)

[6] INO80 requires a polycomb subunit to regulate the establishment of poised chromatin in murine spermatocytes [J].

Chakraborty, Prabuddha ;

Magnuson, Terry .

DEVELOPMENT, 2022, 149 (01)

[7] Loss of function mutations in the rice chromomethylase OsCMT3a cause a burst of transposition [J].

Cheng, Chaoyang ;

Tarutani, Yoshiaki ;

Miyao, Akio ;

Ito, Tasuku ;

Yamazaki, Muneo ;

Sakai, Hiroaki ;

Fukai, Eigo ;

Hirochika, Hirohiko .

PLANT JOURNAL, 2015, 83 (06) :1069-1081

[8] The Biology of Chromatin Remodeling Complexes [J].

Clapier, Cedric R. ;

Cairns, Bradley R. .

ANNUAL REVIEW OF BIOCHEMISTRY, 2009, 78 :273-304

[9] Deposition of Histone Variant H2A.Z within Gene Bodies Regulates Responsive Genes [J].

Coleman-Derr, Devin ;

Zilberman, Daniel .

PLOS GENETICS, 2012, 8 (10)

[10] The INO80 chromatin remodeling complex in transcription, replication and repair [J].

Conaway, Ronald C. ;

Conaway, Joan Weliky .

TRENDS IN BIOCHEMICAL SCIENCES, 2009, 34 (02) :71-77

← 1 2 3 4 5 6 7 8 →