Identification of drought stress-responsive genes in Korean fir (Abies koreana) through comparative RNA-seq analysis

被引：1

作者：

Park, Hyeong Cheol ^{[1
]}

Hwang, Jung Eun ^{[2
]}

机构：

[1] Natl Inst Ecol, Bur Conservat Res, Div Ecol Applicat Res, Ecol Technol Res Team, Seocheon 33657, South Korea

[2] Natl Inst Ecol, Res Ctr Endangered Species, Div Restorat Res, Yeongyang 36531, South Korea

来源：

PLANT BIOTECHNOLOGY REPORTS | 2024年 / 18卷 / 07期

关键词：

<italic>Abies koreana</italic>; Drought stress; Gene ontology classification; Transcription factor; Transcriptomic analysis;

D O I：

10.1007/s11816-024-00941-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Abies koreana, an endangered species sensitive to drought, faces severe decline due to climate change-induced water deficit. In this study, we performed a comprehensive transcriptomic analysis of A. koreana under drought stress. RNA-seq data analysis revealed 85,403 contigs of extended lengths, indicative of a high-quality assembly. Gene Ontology classification revealed strong drought stress-responsive regulation of unigenes associated with various biological processes, cellular components, and molecular functions. Gene expression levels showed variation at different time points, indicating a nuanced plant response. Numerous transcription factors, notably those belonging to ERF, MYB, LBD, and NAC families, were identified, underscoring their critical roles in drought tolerance. Finally, qRT-PCR validation confirmed the reliability of RNA-seq data. Overall, this study reveals candidate genes required for drought tolerance in A. koreana, thus supporting future tree breeding programs for effective forest management.

引用

页码：927 / 938

页数：12

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