Identification and characterization of a novel reovirus strain isolated fromgrass carp (Ctenopharyngodon idella)

被引：1

作者：

Kong, Weiguang ^{[1
]}

Ding, Guangyi ^{[1
]}

Zhang, Qiushi ^{[1
]}

Yuan, Xinjie ^{[1
]}

Zhu, Yuchao ^{[1
]}

Ma, Liyuan ^{[1
]}

Cai, Chang ^{[1
]}

Shi, Yong ^{[1
]}

Zhang, Qianqian ^{[1
]}

Xu, Zhen ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Breeding Biotechnol & Sustainable Aq, Wuhan 430072, Peoples R China

来源：

VIROLOGY JOURNAL | 2025年 / 22卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Grass carp reovirus; Identification; Genome; Pathogenicity; Phylogenetic analysis; COMPLETE GENOME SEQUENCE; GRASS CARP; DIFFERENT GENOTYPES; GCRV;

D O I：

10.1186/s12985-025-02716-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background Grass carp (Ctenopharyngodon idella) hemorrhagic disease (GCHD) is a devastating disease that leads to substantial economic losses in the freshwater aquaculture industry. Results In this study, we investigated an outbreak of GCHD in large-scale grass carp and identified GCRV-II infection. Notably, hematoxylin and eosin (H&E) staining showed severe histopathological changes in the spleen, head kidney, gill, and gut. Furthermore, we sequenced the entire genome of the viral isolate, and multiple sequence alignment and phylogenetic tree analysis indicated that it represents a novel strain of GCRV-II, provisionally named GCRV-YX246. Finally, artificial infection experiments confirmed the strong virulence, high mortality, and severe pathological damage caused by GCRV-YX246, as demonstrated through artificial infection. Conclusions A novel reovirus from large-scale grass carp cultured in China was identified. The discovery of this novel GCRV-II strain enhances our understanding of GCRV-II biology and provides valuable insights for developing more effective prevention strategies for GCHD.

引用

页数：14

共 35 条

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