Determination of pyrroloquinoline quinone disodium salt in milk drink by solid phase extraction-high performance liquid chromatography

被引：0

作者：

Dai, Kun ^{[1
]}

Gong, Pixue ^{[1
]}

Zhao, Yin ^{[1
]}

Xue, Xia ^{[1
]}

Ding, Yi ^{[1
]}

Cai, Ruiqi ^{[1
]}

Xu, Dawei ^{[1
]}

Cui, Yuhua ^{[1
]}

Wang, Jun ^{[1
]}

Hu, Mei ^{[1
]}

Liu, Yanming ^{[1
]}

机构：

[1] Shandong Institute for Food and Drug Control, Key Laboratory of Supervising Technology for Meat and Meat Products for State Market Regulation, Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes,

来源：

Chinese Journal of Analysis Laboratory | 2025年 / 44卷 / 02期

关键词：

Aspartame - Chromatographic analysis - Column chromatography - High performance liquid chromatography;

D O I：

10.13595/j.cnki.issn1000-0720.2023110602

中图分类号：

学科分类号：

摘要：

A method for the determination of pyrroloquinoline quinone disodium salt(PQQ-2Na)in milk drink was established by high performance liquid chromatography(HPLC)coupled with solid phase extraction columns. Samples were extracted using an acetonitrile-citric acid solution(pH 5.0)and purified with an Oasis WAX hybrid weak anion solid phase extraction column. The separation was performed on a Poroshell 120 EC-C18 chromatographic column with a gradient elution using methanol and 20 mmol/L ammonium formate solution as the mobile phase. The target compound was detected by HPLC with fluorescence detector(FLD),and quantification was achieved by the external standard method. The extraction solvents and pre-treatment processes,including purification and concentration, were optimized. The separation efficiency of different chromatographic columns and mobile phase conditions were investigated. The results indicated that PQQ-2Na showed a good linear relationship in the range of 0.10-50 µg/mL, with the correlation coefficient of 0.9992. The detection limit of PQQ-2Na was 0.3 mg/kg,and the quantification limit was 1 mg/kg. The recoveries were in the range of 80.1%-92.2%,with the relative standard deviations(RSDs)of 1.4%-3.2%. This method is suitable for the detection of PQQ-2Na in milk drink. © 2025, Youke Publishing Co.,Ltd. All rights reserved.

引用

页码：293 / 298