Synthesis of Silver Nanoparticles Using Porella pinnata L. Extract and Evaluation of Biological Activity

Developing an eco-friendly method for producing nanomaterials is an area of significant research and commercial interest owing to its numerous applications in various disciplines. This study used a simple green synthesis approach to produce silver nanoparticles (AgNPs) using Porella pinnata L. aqueous extract. Phytochemical constituents of P. pinnata were identified by Gas Chromatography and Mass Spectroscopy (GC-MS). The optical, structural, and morphological characteristics of the AgNPs were found using UV visible absorption spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), Zeta Potential, and scanning electron microscope (SEM). The AgNPs had an average size of 76-84 nm and were mostly spherical. Considering the antimicrobial and antibiofilm activity results, both aqueous extract and AgNP have significant activities against different microorganisms based on the disc diffusion, broth microdilution, and antibiofilm methods. The P. pinnata and AgNPs extracts were significantly inhibited all test culture especially Acinetobacter baumanii ATCC 19606. The agarose gel electrophoresis method showed that extract and AgNP both cleaved DNA by hydrolytic and oxidative. Although both P. pinnata and AgNP extracts have dose-dependent antioxidant activity, AgNP is more effective. Thus, green synthesis AgNPs may be a new alternative therapeutic agent for infection therapy.