Single-cell morphometrics reveals T-box gene-dependent patterns of epithelial tension in the Second Heart field

被引:0
|
作者
Guijarro, Clara [1 ,2 ,3 ]
Song, Solene [2 ,3 ]
Aigouy, Benoit [1 ]
Clement, Raphael [1 ]
Villoutreix, Paul [2 ,3 ]
Kelly, Robert G. [1 ]
机构
[1] Aix Marseille Univ, Turing Ctr Living Syst, CNRS UMR 7288, IBDM, Marseille, France
[2] Aix Marseille Univ, Turing Ctr Living Syst, LIS, UMR 7020, Marseille, France
[3] Aix Marseille Univ, Turing Ctr Living Syst, MMG, Inserm U1251, Marseille, France
关键词
OUTFLOW TRACT; HIPPO PATHWAY; TBX1; POLARITY; GROWTH; VARIABILITY; EXPRESSION; YAP/TAZ; DOMAIN; ROLES;
D O I
10.1038/s41467-024-53612-8
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The vertebrate heart tube extends by progressive addition of epithelial second heart field (SHF) progenitor cells from the dorsal pericardial wall. The interplay between epithelial mechanics and genetic mechanisms during SHF deployment is unknown. Here, we present a quantitative single-cell morphometric analysis of SHF cells during heart tube extension, including force inference analysis of epithelial stress. Joint spatial Principal Component Analysis reveals that cell orientation and stress direction are the main parameters defining apical cell morphology and distinguishes cells adjacent to the arterial and venous poles. Cell shape and mechanical forces display a dynamic relationship during heart tube formation. Moreover, while the T-box transcription factor Tbx1 is necessary for cell orientation towards the arterial pole, activation of Tbx5 in the posterior SHF correlates with the establishment of epithelial stress and SHF deletion of Tbx5 relaxes the progenitor epithelium. Integrating findings from cell-scale feature patterning and mechanical stress provides new insights into cardiac morphogenesis. The embryonic heart tube undergoes elongation via the addition of progenitors from the second heart field, though how epithelial mechanics and genetics interact during this process remains unknown. Here they use quantitative morphometric analysis to reveal that Tbx5 regulates epithelial tension in the posterior region of the second heart field.
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页数:14
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