Unveiling Genetic Diversity Among Bacterial Isolates Using SCoT Markers

被引:1
|
作者
Tekin, Fatih [1 ]
Altin, Irem [2 ]
Aydin, Furkan [3 ]
Alkan, Mehtap [1 ]
Orel, Didem Canik [4 ]
Ardic, Mustafa [3 ]
Gasic, Katarina [5 ]
Kovacs, Tamas [6 ]
Allen, Caitilyn [7 ]
Ozer, Goeksel [1 ]
机构
[1] Bolu Abant Izzet Baysal Univ, Fac Agr, Dept Plant Protect, TR-14030 Bolu, Turkiye
[2] Duzce Univ, Fac Agr, Dept Plant Protect, TR-81620 Duzce, Turkiye
[3] Aksaray Univ, Fac Engn, Dept Food Engn, TR-68100 Aksaray, Turkiye
[4] Ankara Univ, Dept Plant Protect, Fac Agr, TR-06110 Ankara, Turkiye
[5] Inst Plant Protect & Environm, Teodora Drajzera 9, Belgrade 11040, Serbia
[6] Enviroinvest Corp, Nanophagetherapy Ctr, Kertvaros U2, H-7632 Pecs, Hungary
[7] Univ Wisconsin Madison, Madison, WI USA
关键词
PCR;
D O I
10.1007/s00284-024-03938-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This study aimed to unveil the genetic diversity among 47 bacterial isolates from various species using start codon targeted (SCoT) markers. Six SCoT primers yielded 219 reproducible bands, with 89.04% exhibiting polymorphism. The amplification process generated 28 to 50 fragments per primer, with an average of 36.50. Genetic diversity was quantified using polymorphic information content (PIC) values ranging from 0.11 to 0.14, with SCoT32 showing the highest PIC (0.14) and SCoT23 the lowest (0.11). The resolving power (RP) index, used to assess primer discriminatory power, varied significantly, with SCoT23 demonstrating the highest RP (6.00) and SCoT29 the lowest (4.51). Comparative analysis with conventional markers like M13 and (GTG)5 revealed that certain SCoT primers exhibited superior PIC values, which indicates enhanced utility for interspecies differentiation. The high discrimination level achieved by SCoT primers underscores their effectiveness in genetic differentiation and biodiversity assessment within bacterial populations. This research highlights SCoT markers as powerful tools for microbial genetic studies, which offers valuable insights into bacterial diversity and provides a robust methodological framework for future investigations aimed at elucidating genetic variation and improving species identification. The application of SCoT markers represents a significant advancement in molecular techniques for bacterial characterization and phylogenetic analysis, demonstrating their potential to enhance our understanding of microbial genetics and evolution.
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页数:8
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