Siglec-15 is a putative receptor for porcine epidemic diarrhea virus infection

被引：0

作者：

Feng, Zhihua ^{[1
,2
,3
,4
]}

Fu, Yajuan ^{[3
,4
]}

Yang, Sheng ^{[3
,4
]}

Zhao, Heng ^{[3
,4
]}

Lin, Minhua ^{[3
,4
]}

Liu, Chuancheng ^{[3
,4
]}

Huang, Weili ^{[3
,4
]}

He, Xinyan ^{[3
,4
]}

Chen, Yao ^{[3
,4
]}

Chen, Jianxin ^{[1
,2
]}

Shen, Yangkun ^{[3
,4
]}

Li, Zhaolong ^{[5
]}

Chen, Qi ^{[1
,2
,3
,4
]}

机构：

[1] Fujian Normal Univ, Key Lab Optoelect Sci & Technol Med, Minist Educ, Fuzhou 350117, Fujian, Peoples R China

[2] Fujian Normal Univ, Coll Photon & Elect Engn, Fuzhou 350117, Fujian, Peoples R China

[3] Fujian Normal Univ, Biomed Res Ctr South China, Fujian Key Lab Innate Immune Biol, Qishan Campus, Fuzhou 350117, Fujian, Peoples R China

[4] Fujian Normal Univ, Coll Life Sci, Qishan Campus, Fuzhou 350117, Fujian, Peoples R China

[5] Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Fujian, Peoples R China

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 2025年 / 82卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Swine alpha-coronaviruses; Host factor; Mouse model; Antibody drug; Cross-species transmission; RESPIRATORY SYNDROME VIRUS; CELL ENTRY; PROTEIN; ACTIVATION; PROMOTES; SPIKE; PEDV; COINFECTION; PIGLETS; TRYPSIN;

D O I：

10.1007/s00018-025-05672-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Porcine epidemic diarrhea virus (PEDV) has caused significant losses in the pork industry, but the mechanism of PEDV infection is still unclear. On the basis of our RNA-Seq data and due to the potential role of sialic acid as a coreceptor, we investigated the function of sialic acid-binding Ig-like lectin 15 (Siglec-15) to determine its role as a receptor in PEDV infection. We found that Siglec-15 enhances PEDV infection by promoting viral adsorption to host cells. Coimmunoprecipitation and immunofluorescence assays revealed that Siglec-15 binds to the S1 subunit and M protein of PEDV. PEDV infectivity was significantly reduced in Siglec-15 knockout mice. In addition, we developed a monoclonal antibody targeting Siglec-15 that can effectively inhibit PEDV infection both in vitro and in vivo. Overall, our study suggests that Siglec-15 may be a receptor for PEDV infection, which is important for related mechanistic studies and reveals a novel target for anti-PEDV therapeutic development.

引用

页数：22

共 84 条

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