Roles of miR-20a-5p in breast cancer based on the clinical and multi-omic (CAMO) cohort and in vitro studies

被引:0
|
作者
Tylden, Eline Sol [1 ]
Delgado, Andre Berli [1 ]
Lukic, Marko [2 ]
Moi, Line [3 ]
Busund, Lill-Tove Rasmussen [1 ,3 ]
Pedersen, Mona Irene [4 ]
Lombardi, Ana Paola [1 ]
Olsen, Karina Standahl [2 ]
机构
[1] UiT Arctic Univ Norway, Fac Hlth Sci, Dept Med Biol, Tromso, Norway
[2] UiT Arctic Univ Norway, Fac Hlth Sci, Dept Community Med, Tromso, Norway
[3] Univ Hosp North Norway, Dept Clin Pathol, Tromso, Norway
[4] UiT Arctic Univ Norway, Fac Hlth Sci, Dept Clin Med, Tromso, Norway
来源
SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期
关键词
Breast cancer; miR-20a-5p; microRNA; miRNA; Biomarker; Epidemiology; Tissue microarray; In situ hybridization; Translational research; NORWEGIAN WOMEN; GENE-EXPRESSION; MICRORNAS; CELLS; TRANSCRIPTION; RECEPTOR; GROWTH;
D O I
10.1038/s41598-024-75557-0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
MicroRNAs are involved in breast cancer development and progression, holding potential as biomarkers and therapeutic targets or tools. The roles of miR-20a-5p, a member of the oncogenic miR-17-92 cluster, remain poorly understood in the context of breast cancer. In this study, we elucidate the role of miR-20a-5p in breast cancer by examining its associations with breast cancer risk factors and clinicopathological features, and its functional roles in vitro. Tissue microarrays from 313 CAMO cohort breast cancer surgical specimens were constructed, in situ hybridization was performed and miR-20a-5p expression was semiquantitatively scored in tumor stromal fibroblasts, and in the cytoplasm and nuclei of cancer cells. In vitro analysis of the effect of miR-20a-5p transfection on proliferation, migration and invasion was performed in three breast cancer cell lines. High stromal miR-20a-5p was associated with higher Ki67 expression, and higher odds of relapse, compared to low expression. Compared to postmenopausal women, women who were premenopausal at diagnosis had higher odds of high stromal and cytoplasmic miR-20a-5p expression. Cytoplasmic miR-20a-5p was significantly associated with tumor grade. In tumors with high cytoplasmic miR-20a-5p expression compared to low expression, there was a tendency towards having a basal-like subtype and high Ki67. In contrast, high nuclear miR-20a-5p in cancer cells was associated with smaller tumor size and lower odds of lymph node metastasis, compared to low nuclear expression. Transfection with miR-20a-5p in breast cancer cell lines led to increased migration and invasion in vitro. While the majority of our results point towards an oncogenic role, some of our findings indicate that the associations of miR-20a-5p with breast cancer related risk factors and outcomes may vary based on tissue- and subcellular location. Larger studies are needed to validate our findings and further investigate the clinical utility of miR-20a-5p.
引用
收藏
页数:18
相关论文
共 50 条
  • [1] Cohort profile: The Clinical and Multi-omic (CAMO) cohort, part of the Norwegian Women and Cancer (NOWAC) study
    Delgado, Andre Berli
    Tylden, Eline Sol
    Lukic, Marko
    Moi, Line
    Busund, Lill-Tove Rasmussen
    Lund, Eiliv
    Olsen, Karina Standahl
    PLOS ONE, 2023, 18 (02):
  • [2] Regulatory mechanism of miR-20a-5p expression in Cancer
    Wei Huang
    Xiaoyue Wu
    Shuaixi Xiang
    Mingxin Qiao
    Xiao Cen
    Xuefeng Pan
    Xinqi Huang
    Zhihe Zhao
    Cell Death Discovery, 8
  • [3] Regulatory mechanism of miR-20a-5p expression in Cancer
    Huang, Wei
    Wu, Xiaoyue
    Xiang, Shuaixi
    Qiao, Mingxin
    Cen, Xiao
    Pan, Xuefeng
    Huang, Xinqi
    Zhao, Zhihe
    CELL DEATH DISCOVERY, 2022, 8 (01)
  • [4] Triple-negative and luminal A breast tumors: differential expression of miR-18a-5p, miR-17-5p, and miR-20a-5p
    Cabral Calvano Filho, Carlos Marino
    Calvano-Mendes, Daniele Carvalho
    Carvalho, Katia Candido
    Maciel, Gustavo Arantes
    Ricci, Marcos Desiderio
    Torres, Ana Paula
    Filassi, Jose Roberto
    Baracat, Edmund Chada
    TUMOR BIOLOGY, 2014, 35 (08) : 7733 - 7741
  • [5] miR-20a-5p inhibits endometrial cancer progression by targeting janus kinase 1
    He, Ying
    Ma, Hui
    Wang, Jing
    Kang, Yanhua
    Xue, Qianlong
    ONCOLOGY LETTERS, 2021, 21 (05)
  • [6] Investigating Deep Learning Based Breast Cancer Subtyping Using Pan-Cancer and Multi-Omic Data
    Cristovao, Francisco
    Cascianelli, Silvia
    Canakoglu, Arif
    Carman, Mark
    Nanni, Luca
    Pinoli, Pietro
    Masseroli, Marco
    IEEE-ACM TRANSACTIONS ON COMPUTATIONAL BIOLOGY AND BIOINFORMATICS, 2022, 19 (01) : 121 - 134
  • [7] Low expression of miR-20a-5p predicts benefit to bevacizumab in metastatic breast cancer patients treated within the TANIA trial
    Gampenrieder, S. P. P.
    Rinnerthaler, G.
    Hackl, H.
    Steiner, M.
    Fuentes, C. Monzo
    Hufnagl, C.
    Hauser-Kronberger, C.
    Egle, A.
    Greil, R.
    ANNALS OF ONCOLOGY, 2017, 28
  • [8] Breast cancer cell-derived exosomal miR-20a-5p promotes the proliferation and differentiation of osteoclasts by targeting SRCIN1
    Guo, Ling
    Zhu, Ye
    Li, Liandi
    Zhou, Shufen
    Yin, Guohua
    Yu, Guanghao
    Cui, Hujun
    CANCER MEDICINE, 2019, 8 (12): : 5687 - 5701
  • [9] Overexpression of miR-20a-5p in Tumor Epithelium Is an Independent Negative Prognostic Indicator in Prostate Cancer-A Multi-Institutional Study
    Stoen, Maria J.
    Andersen, Sigve
    Rakaee, Mehrdad
    Pedersen, Mona, I
    Ingebriktsen, Lise M.
    Donnem, Tom
    Lombardi, Ana P. G.
    Kilvaer, Thomas K.
    Busund, Lill-Tove R.
    Richardsen, Elin
    CANCERS, 2021, 13 (16)
  • [10] MiR-20a-5p represses the multi-drug resistance of osteosarcoma by targeting the SDC2 gene
    Zhao, Fangfang
    Pu, Youguang
    Cui, Mingda
    Wang, Haiyan
    Cai, Shanbao
    CANCER CELL INTERNATIONAL, 2017, 17