A capillary-driven microfluidic device for performing spatial multiplex PCR

被引：0

作者：

Wiederkehr, Rodrigo S. ^{[1
]}

Marchal, Elisabeth ^{[1
]}

Fauvart, Maarten ^{[1
]}

Forceville, Tomas ^{[1
]}

Taher, Ahmed ^{[1
]}

Steylaerts, Tim ^{[1
]}

Choe, Youngjae ^{[1
]}

Dusar, Hans ^{[1
]}

Lenci, Silvia ^{[1
]}

Siouti, Eleni ^{[2
]}

Potsika, Vassiliki T. ^{[3
]}

Andreakos, Evangelos ^{[2
]}

Stakenborg, Tim ^{[1
]}

机构：

[1] IMEC, Kapeldreef 75, B-3001 Leuven, Belgium

[2] BRFAA Biomed Res Fdn Acad Athens, 4 Soranou Ephessiou St, Athens, Greece

[3] Univ Ioannina, Unit Med Technol & Intelligent Informat Syst, Ioannina, Greece

来源：

BIOMEDICAL MICRODEVICES | 2025年 / 27卷 / 02期

基金：

欧盟地平线“2020”;

关键词：

Capillary flow; Multiplex PCR; Silicon chip; Lab-on-a-chip; Fluorescence detection; REAL-TIME PCR; SYSTEM; CHIP;

D O I：

10.1007/s10544-025-00745-2

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Multiplex polymerase chain reaction (PCR) tests multiple biomarkers or pathogens that cause overlapping symptoms, making it an essential tool in syndromic testing. To achieve a multiplex PCR on chip, a design based on capillary-driven fluidic actuation is proposed. Our silicon chip features 22 reaction chambers and allows primers and probes to be pre-spotted in the reaction chambers prior to use. The design facilitates rapid sample loading through a common inlet channel, delivering reagents to all reaction chambers in less than 10 s. A custom clamping mechanism combined with a double depth cavity design ensures proper sealing during temperature cycling without the need for extra reagents like oil. Temperature cycling and fluorescence imaging were performed using custom-made hardware. As a proof of concept, two single nucleotide polymorphisms (SNPs), CyP2C19*2 and PCSK9 were detected. These results demonstrate the feasibility of on-chip multiplex PCR, compatible with different assays in parallel and requiring only a single pipetting step for reagent loading, without active fluidic actuation like pumping.

引用

页数：11

共 52 条

[1]

Ahmed W., 2021, Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance, DOI DOI 10.20944/PREPRINTS202104.0481.V1

[2] Polymerase chain reaction in microfluidic devices [J].