A pyrene-based fluorescent probe for H2S detection and cellular imaging

被引:0
|
作者
Alam, Said [1 ]
Tao, Xuanzuo [1 ]
Mao, Yanxia [1 ]
Zheng, Shaojun [1 ]
Jiang, Chunhui [1 ]
Chen, Shu-Yang [2 ]
Lu, Hongfei [1 ]
机构
[1] Jiangsu Univ Sci & Technol, Sch Environm & Chem Engn, Zhenjiang 212000, Peoples R China
[2] Nanyang Normal Univ, Coll Chem & Pharmaceut Engn, Nanyang 473061, Peoples R China
基金
中国国家自然科学基金;
关键词
Pyrene; Fluorescent probe; H2S detection; Hele cell; Spectroscopy; LARGE STOKES SHIFT; HYDROGEN-SULFIDE; SELECTIVE DETECTION; LIVING CELLS; NAKED-EYE; MITOCHONDRIA; INFLAMMATION; CHEMOSENSOR; IONS;
D O I
10.1007/s43630-025-00695-x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hydrogen sulfide (H2S) is a significant reactive sulfur species (RSS) involved in various human diseases, also playing an important role in many physiological and pathological processes. Thus, the development of an effective method for detecting H2S in mammalian cells is of great importance. In this study, we present the synthesis of a novel pyrene-based fluorescent probe, DPP, specifically designed for the selective detection of H2S. The DPP exhibits remarkable sensitivity, with a low detection limit of 0.63 mu M, and demonstrates high selectivity for H2S in the presence of various interfering species. Additionally, the probe has demonstrated rapid detection of H2S in less than 6 min. The detection mechanism was thoroughly validated using H-1 NMR, FT-IR, UV and fluorescence spectra. Moreover, the applicability of DPP was successfully demonstrated in both in vitro and in vivo settings using HeLa cells, confirming its potential as a powerful tool for monitoring H2S in biological systems. Additionally, the probe exhibited excellent performance in detecting H2S in water samples and in paper strip-based assays, further highlighting its versatility and practical utility for environmental monitoring and on-site applications. [GRAPHICS]
引用
收藏
页码:405 / 415
页数:11
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