Optimization of green ultrasound-assisted extraction of phenolic compounds from Crataegus laciniata leaves and assessing for antioxidant activity, enzyme inhibition, and UPLC-ESI-MS-MS guided identification of metabolites

被引：1

作者：

Saidene, Naima ^{[1
]}

Chaher-Bazizi, Nassima ^{[1
]}

Kadi, Radia ^{[1
]}

Atmani-Kilani, Dina ^{[1
]}

Atmani, Djebbar ^{[1
]}

Zaidi, Sidali ^{[1
]}

Kaddour, Taous ^{[1
]}

Rahmani-Berboucha, Meriem ^{[1
]}

Debbache-Benaida, Nadjet ^{[1
]}

Bouadam, Said ^{[2
]}

Farhi-Bouadam, Baya ^{[3
]}

Bachir-bey, Mostapha ^{[1
]}

机构：

[1] Univ Bejaia, Fac Sci Nat & Vie, Lab Biochim Appl, Bejaia 06000, Algeria

[2] Univ Bejaia, Fac Sci Nat & Vie, Bejaia 06000, Algeria

[3] Univ Bejaia, Fac Sci Nat & Vie, Lab Ecol & Environm, Bejaia 06000, Algeria

来源：

CHEMICAL PAPERS | 2024年 / 78卷 / 18期

关键词：

Crataegus laciniata; Ultrasound-assisted extraction; Box-Behnken model; Enzyme inhibition; Antioxidant activities; HELICOBACTER-PYLORI; STRUCTURAL-CHARACTERIZATION; ELECTROSPRAY-IONIZATION; UREASE INHIBITORS; FLAVONOIDS; PINNATIFIDA; RECOVERY; PLASMA; FRUITS; ASSAY;

D O I：

10.1007/s11696-024-03745-3

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The wide therapeutic potential attributed to Crataegus laciniata leaves has spurred considerable interest, yet comprehensive studies on the extraction of bioactive compounds, phytochemical composition, and biological properties are scarce. The present study aimed at optimizing ultrasound-assisted extraction of phenolic compounds from C. laciniata leaves (CLL), and assessing their composition, antioxidant potential, and enzyme inhibition. Box-Behnken design was used to estimate the effects of ethanol concentration, time, and temperature on the extraction procedure. Phytochemical composition was analyzed using UPLC-ESI-MS-MS, operating in the multiple reaction monitoring (MRM) mode. Biological properties were tested through antioxidant activity and inhibition potential of three key enzymes implicated in different pathologies. The mathematical model for total phenolic content (TPC) recovery indicated high significance and experimental adequacy with optimal extraction conditions of 51.18%, 54.82 degrees C, and 35.80 min for ethanol concentration, temperature, and time, respectively. Under these conditions the TPC was 308.25 mg GAE/g. UPLC-ESI-MS-MS analysis led to the identification of 15 phenolic compounds, including 9 flavonoids (quercetin, myricetin, rutin, hesperetin, naringenin, chrysin, vitexin, catechin, and esculin) and 6 phenolic acids (chlorogenic, gallic, ferulic, cinnamic, coumaric and 2-methoxybenzoic acids). The CLL extract exhibited strong antioxidant activity revealed by DPPH, ABTS, OH free radical scavenging activities and the FRAP assay. In addition, the optimal extract showed a fairly good inhibitory potential against urease, alpha-glycosidase, and acetylcholinesterase, with IC(50 )values of 133.28, 7.21, and 153.94 mu g/mL, respectively. The optimal extract of C. laciniata leaves can be considered a rich and diversified source of phenolic compounds with potent biological activities with great potential for exploration in the pharmacological and food industries.

引用

页码：9325 / 9339

页数：15

共 95 条

[11] Chemical composition, antioxidant and antibacterial activity of Crataegus monogyna leaves' extracts [J].

Belabdelli, Fouzia ;

Bekhti, Nabila ;

Piras, Alessandra ;

Benhafsa, Fouad Mekhalef ;

Ilham, Mokhtari ;

Adil, Selka ;

Anes, Lazzaz .

NATURAL PRODUCT RESEARCH, 2022, 36 (12) :3234-3239

[12] Ultrasonic-assisted extraction of total phenolic contents from Phoenix dactylifera and evaluation of antioxidant activity: statistical optimization of extraction process parameters [J].

Benkerrou, Fatiha ;

Bey, Mostapha Bachir ;

Amrane, Meriem ;

Louaileche, Hayette .

JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION, 2018, 12 (03) :1910-1916

[13] The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay [J].

Benzie, IFF ;

Strain, JJ .

ANALYTICAL BIOCHEMISTRY, 1996, 239 (01) :70-76

[14] Electrospray ionisation mass spectrometric behaviour of flavonoid 5-O-glucosides and their positional isomers detected in the extracts from the bark of Prunus cerasus L. and Prunus avium L. [J].

Beszterda, Monika ;

Franski, Rafal .

PHYTOCHEMICAL ANALYSIS, 2021, 32 (03) :433-439

[15] Optimization of Phenolic Compound Recovery and Antioxidant Activity of Light and Dark Dried Fig (Ficus carica L.) Varieties [J].

Bey, Mostapha Bachir ;

Louaileche, Hayette ;

Zemouri, Saliana .

FOOD SCIENCE AND BIOTECHNOLOGY, 2013, 22 (06) :1613-1619

[16]

Caliskan O., 2015, The mediterranean diet, P621, DOI [10.1016/B978-0-12-407849-9.00055-6, DOI 10.1016/B978-0-12-407849-9.00055-6, DOI 10.1016/B978-0-12-407849-9.00055-5]

[17] Response Surface Methodology to Optimize the Extraction of Carotenoids from Horticultural By-Products-A Systematic Review [J].

Cano-Lamadrid, Marina ;

Martinez-Zamora, Lorena ;

Mozafari, Laleh ;

Bueso, Maria Carmen ;

Kessler, Mathieu ;

Artes-Hernandez, Francisco .

FOODS, 2023, 12 (24)

[18] Optimization of ultrasound-assisted extraction of anthocyanins from haskap berries (Lonicera caerulea L.) using Response Surface Methodology [J].

Celli, Giovana Bonat ;

Ghanem, Amyl ;

Brooks, Marianne Su-Ling .

ULTRASONICS SONOCHEMISTRY, 2015, 27 :449-455

[19] Green extraction of natural products. Origins, current status, and future challenges [J].

Chemat, Farid ;

Abert-Vian, Maryline ;

Fabiano-Tixier, Anne Sylvie ;

Strube, Jochen ;

Uhlenbrock, Lukas ;

Gunjevic, Veronika ;

Cravotto, Giancarlo .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2019, 118 :248-263

[20] Applications of ultrasound in food technology: Processing, preservation and extraction [J].

Chemat, Farid ;

Zill-e-Huma ;

Khan, Muhammed Kamran .

ULTRASONICS SONOCHEMISTRY, 2011, 18 (04) :813-835

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