Fluoridated silver nanocomposites for caries management: an in-vitro assessment of the cytological and antibacterial profiles

被引：0

作者：

Essawy, Marwa M. ^{[1
,2
]}

Al Achy, Samar N. ^{[2
,3
]}

Talaat, Dalia M. ^{[4
]}

El-Tekeya, Magda M. ^{[4
]}

Essa, Sara ^{[5
]}

Nabil, Nouran ^{[4
]}

Ammar, Nour ^{[4
]}

机构：

[1] Alexandria Univ, Fac Dent, Dept Oral Pathol, Alexandria 21521, Egypt

[2] Alexandria Univ, Fac Med, CERRMA, Alexandria 21521, Egypt

[3] Alexandria Univ, Fac Med, Dept Pathol, Alexandria 21521, Egypt

[4] Alexandria Univ, Fac Dent, Dept Pediat Dent & Dent Publ Hlth, Alexandria 21521, Egypt

[5] Alexandria Univ, Fac Med, Dept Med Microbiol & Immunol, Alexandria 21521, Egypt

来源：

BMC ORAL HEALTH | 2025年 / 25卷 / 01期

关键词：

Cytotoxicity; Gallic acid; Minimum inhibitory concentration; Nano silver; Polyethylene glycol; Streptococcus mutans; CONTAINING PRODUCTS CYTOTOXICITY; METAL-ION RELEASE; ORTHODONTIC BRACKETS; NANOPARTICLES; CELLS; SURFACE; COMBINATION; MECHANISMS; STABILITY; CORROSION;

D O I：

10.1186/s12903-025-05691-2

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

BackgroundSilver nanoparticles (AgNPs) have antibacterial properties with potential applications in managing dental caries. Functionalization with fluoride may further enhance AgNPs' antibacterial efficacy. This study evaluated the impact of fluoridated AgNPs coated with various surface moieties on their safety profile and antibacterial effects against cariogenic bacteria as a potential anti-cariogenic treatment.MethodsAgNP synthesis followed citrate and gallic acid reduction methods with polyethylene glycol (PEG) and polyvinylpyrrolidone coating. Functionalizing AgNPs with sodium fluoride (NaF) proceeded. Testing the safety of synthesized compounds was done on human gingival fibroblasts and oral epithelial cells. Meanwhile, minimum inhibitory concentration (MIC) determination against Streptococcus mutans was executed to verify antibacterial activity.ResultsGallic-reduced AgNPs revealed higher yielding capacity than citrate-AgNPs. Cytologically, PEGylation reinforced citrate-AgNPs stability and improved IC50 range up to similar to 4.2 x 1016 mu g/mL and 64.3 mu g/mL on fibroblastic and epithelial lineages. PEGylated AgNPs counteracted the cytotoxicity of free NaF with antagonistic combinational effect of NaF@PEG gallic-AgNPs on gingival fibroblasts. Microbiologically, AgNPs recorded an enhanced antimicrobial activity of similar to 5.3 +/- 2.3 mu g/mL averaged MIC against Streptococcus mutans. Furthermore, fluoridation of PEG gallic-AgNPs depicted an additive antimicrobial propensity.ConclusionsThis dual action nanoplatform successfully integrates fluoride and silver components, reducing fluoride concentrations to safety range while maximizing silver's antibacterial properties. Engineered NaF@PEGylated nanosilver formulation represents promising anti-cariogenic strategy that optimizes therapeutic efficacy while maintaining biological safety.

引用

页数：14