EFNB1 drives glioma progression and shapes the immune microenvironment: a potential prognostic biomarker

被引:0
作者
Zheng, Yungui [1 ]
Shi, Jiasong [1 ]
机构
[1] Shantou Univ, Affiliated Hosp 1, Med Coll, Dept Neurosurg, 57 Changping Rd, Shantou, Peoples R China
关键词
EFNB1; Tumor immune microenvironment; Glioma; Prognosis; Biomarker; CELLS;
D O I
10.1007/s12672-025-01867-y
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Gliomas, a heterogeneous group of tumors affecting the brain and spinal cord, present a significant clinical challenge. Ephrin B1 (EFNB1) has been implicated in various malignancies. However, its role in gliomas remained poorly understood. Hence, this study aimed to elucidate the connection between EFNB1 and the progression of glioma. A retrospective RNA-seq analysis was conducted by utilizing the data from glioma patients in the TCGA and CGGA databases. Kaplan-Meier survival analysis and multivariate regression models were employed to evaluate the prognostic significance of EFNB1. RT-PCR was used to quantify EFNB1 expression in glioma tissues and cell lines. Meanwhile, in vitro assays were carried out to assess its functional roles in glioma cells. Our findings demonstrated that EFNB1 expression was significantly elevated in gliomas and other cancers. Moreover, high EFNB1 expression was closely correlated with advanced clinical stages and poor prognosis. Notably, multivariate analysis identified EFNB1 as an independent prognostic factor for overall survival. KEGG pathway analysis suggested that EFNB1 was involved in critical biological processes, including the cell cycle, protein processing in the endoplasmic reticulum, Epstein-Barr virus infection, and Salmonella infection. Furthermore, EFNB1 expression was associated with immune cell infiltration, particularly Th2 cells, macrophages, and plasmacytoid dendritic cells. In glioma cells, EFNB1 expression was markedly increased. Consequently, functional experiments demonstrated that EFNB1 knockdown inhibited glioma cell proliferation, invasion, and migration. These results highlighted EFNB1 as a novel independent prognostic biomarker and suggest its potential role in shaping the immunological microenvironment of gliomas.
引用
收藏
页数:18
相关论文
共 45 条
  • [1] Adult Glioblastoma
    Alexander, Brian M.
    Cloughesy, Timothy F.
    [J]. JOURNAL OF CLINICAL ONCOLOGY, 2017, 35 (21) : 2402 - +
  • [2] Glioma: molecular signature and crossroads with tumor microenvironment
    Barthel, Lennart
    Hadamitzky, Martin
    Dammann, Philipp
    Schedlowski, Manfred
    Sure, Ulrich
    Thakur, Basant Kumar
    Hetze, Susann
    [J]. CANCER AND METASTASIS REVIEWS, 2022, 41 (01) : 53 - 75
  • [3] Glioblastoma Multiforme, Diagnosis and Treatment; Recent Literature Review
    Batash, Ron
    Asna, Noam
    Schaffer, Pamela
    Francis, Nicole
    Schaffer, Moshe
    [J]. CURRENT MEDICINAL CHEMISTRY, 2017, 24 (27) : 3002 - 3009
  • [4] Altered cellular metabolism in gliomas - an emerging landscape of actionable co-dependency targets
    Bi, Junfeng
    Chowdhry, Sudhir
    Wu, Sihan
    Zhang, Wenjing
    Masui, Kenta
    Mischel, Paul S.
    [J]. NATURE REVIEWS CANCER, 2020, 20 (01) : 57 - 70
  • [5] Glioma stem cells and their roles within the hypoxic tumor microenvironment
    Boyd, Nathaniel H.
    Tran, Anh Nhat
    Bernstock, Joshua D.
    Etminan, Tina
    Jones, Amber B.
    Gillespie, G. Yancey
    Friedman, Gregory K.
    Hjelmeland, Anita B.
    [J]. THERANOSTICS, 2021, 11 (02): : 665 - 683
  • [6] Current and future strategies for treatment of glioma
    Bush, Nancy Ann Oberheim
    Chang, Susan M.
    Berger, Mitchel S.
    [J]. NEUROSURGICAL REVIEW, 2017, 40 (01) : 1 - 14
  • [7] Targeting macrophages: therapeutic approaches in cancer
    Cassetta, Luca
    Pollard, Jeffrey W.
    [J]. NATURE REVIEWS DRUG DISCOVERY, 2018, 17 (12) : 887 - 904
  • [8] EphrinB1 Interacts with CNK1 and Promotes Cell Migration through c-Jun N-terminal Kinase (JNK) Activation
    Cho, Hee Jun
    Hwang, Yoo-Seok
    Mood, Kathleen
    Ji, Yon Ju
    Lim, Junghwa
    Morrison, Deborah K.
    Daar, Ira O.
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289 (26) : 18556 - 18568
  • [9] Cell biology-metabolic crosstalk in glioma
    Colquhoun, Alison
    [J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2017, 89 : 171 - 181
  • [10] The Interplay between Glioblastoma and Its Microenvironment
    Dapash, Mark
    Hou, David
    Castro, Brandyn
    Lee-Chang, Catalina
    Lesniak, Maciej S.
    [J]. CELLS, 2021, 10 (09)