Isotropic 100-nm resolution live-cell imaging with 4Pi-SIM

被引：0

作者：

Huang, Xiaoshuai ^{[1
]}

Zhang, Yongdeng ^{[2
]}

机构：

[1] Peking Univ, Beijing, Peoples R China

[2] Westlake Univ, Hangzhou, Peoples R China

来源：

NATURE METHODS | 2025年 / 22卷 / 02期

关键词：

MICROSCOPY;

D O I：

10.1038/s41592-024-02514-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed 4Pi-SIM, which integrates 4Pi microscopy with structured illumination microscopy to achieve isotropic optical resolution through interference in both illumination and detection. We demonstrate its capabilities by time-lapse volumetric imaging of various subcellular structures at 100-nm resolution.

引用

收藏

页码：233 / 234

页数：2

相关论文

共 5 条

[1] Fast, long-term, super-resolution imaging with Hessian structured illumination microscopy [J].

Huang, Xiaoshuai ;

Fan, Junchao ;

Li, Liuju ;

Liu, Haosen ;

Wu, Runlong ;

Wu, Yi ;

Wei, Lisi ;

Mao, Heng ;

Lal, Amit ;

Xi, Peng ;

Tang, Liqiang ;

Zhang, Yunfeng ;

Liu, Yanmei ;

Tan, Shan ;

Chen, Liangyi .

NATURE BIOTECHNOLOGY, 2018, 36 (05) :451-+

[2] Interferometer-based structured-illumination microscopy utilizing complementary phase relationship through constructive and destructive image detection by two cameras [J].

Shao, L. ;

Winoto, L. ;

Agard, D. A. ;

Gustafsson, M. G. L. ;

Sedat, J. W. .

JOURNAL OF MICROSCOPY, 2012, 246 (03) :229-236

[3] I5S:: Wide-field light microscopy with 100-nm-scale resolution in three dimensions [J].

Shao, Lin ;

Isaac, Berith ;

Uzawa, Satoru ;

Agard, David A. ;

Sedat, John W. ;

Gustafsson, Mats G. L. .

BIOPHYSICAL JOURNAL, 2008, 94 (12) :4971-4983

[4]

Shao L, 2011, NAT METHODS, V8, P1044, DOI [10.1038/nmeth.1734, 10.1038/NMETH.1734]

[5] Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging [J].

Zhang, Yongdeng ;

Schroeder, Lena K. ;

Lessard, Mark D. ;

Kidd, Phylicia ;

Chung, Jeeyun ;

Song, Yuanbin ;

Benedetti, Lorena ;

Li, Yiming ;

Ries, Jonas ;

Grimm, Jonathan B. ;

Lavis, Luke D. ;

De Camilli, Pietro ;

Rothman, James E. ;

Baddeley, David ;

Bewersdorf, Joerg .

NATURE METHODS, 2020, 17 (02) :225-+