Label-free live cell recognition and tracking for biological discoveries and translational applications

被引：0

作者：

Biqi Chen ^{[1
]}

Zi Yin ^{[2
]}

Billy Wai-Lung Ng ^{[3
]}

Dan Michelle Wang ^{[4
]}

Rocky S. Tuan ^{[5
]}

Ryoma Bise ^{[1
]}

Dai Fei Elmer Ker ^{[6
]}

机构：

[1] The Chinese University of Hong Kong,Institute for Tissue Engineering and Regenerative Medicine

[2] Zhejiang University,Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, School of Medicine

[3] Zhejiang University,Institute of Sports Medicine

[4] The Chinese University of Hong Kong,School of Pharmacy, Faculty of Medicine

[5] The Chinese University of Hong Kong,Li Ka Shing Institute of Health Sciences, Faculty of Medicine

[6] The Chinese University of Hong Kong,School of Biomedical Sciences, Faculty of Medicine

[7] The Chinese University of Hong Kong,Ministry of Education Key Laboratory for Regenerative Medicine, School of Biomedical Sciences, Faculty of Medicine

[8] The Chinese University of Hong Kong,Department of Orthopaedics and Traumatology, Faculty of Medicine

[9] Hong Kong Science Park,Center for Neuromusculoskeletal Restorative Medicine

[10] Kyushu University,Department of Advanced Information Technology, Faculty of Information Science and Electrical Engineering

[11] The Hong Kong Polytechnic University,Department of Biomedical Engineering, Faculty of Engineering

来源：

npj Imaging | / 2卷 / 1期

关键词：

D O I：

10.1038/s44303-024-00046-y

中图分类号：

学科分类号：

摘要：

Label-free, live cell recognition (i.e. instance segmentation) and tracking using computer vision-aided recognition can be a powerful tool that rapidly generates multi-modal readouts of cell populations at single cell resolution. However, this technology remains hindered by the lack of accurate, universal algorithms. This review presents related biological and computer vision concepts to bridge these disciplines, paving the way for broad applications in cell-based diagnostics, drug discovery, and biomanufacturing.

引用

共 50 条

[1] Low cost label-free live cell imaging for biological samples
Seniya, C.
Towers, C. E.
Towers, D. P.
QUANTITATIVE PHASE IMAGING III, 2017, 10074
[2] Label-free, Live Cell Imaging of Transendothelial Migration
OClair, Belinda
Li, Guangnan
Appledorn, Daniel
JOURNAL OF IMMUNOLOGY, 2016, 196
[3] Advanced Fluorescence and Label-Free Live Cell Microscopy
Huser, Thomas
AOE 2008: ASIA OPTICAL FIBER COMMUNICATION AND OPTOELECTRONIC EXPOSITION AND CONFERENCE, 2009,
[4] Label-free and live cell imaging by interferometric scattering microscopy
Park, Jin-Sung
Lee, Il-Buem
Moon, Hyeon-Min
Joo, Jong-Hyeon
Kim, Kyoung-Hoon
Hong, Seok-Cheol
Cho, Minhaeng
CHEMICAL SCIENCE, 2018, 9 (10) : 2690 - 2697
[5] Label-free optical biosensors for food and biological sensor applications
Khansili, Nishtha
Rattu, Gurdeep
Krishna, Prayaga M.
SENSORS AND ACTUATORS B-CHEMICAL, 2018, 265 : 35 - 49
[6] Label-free biomechanical nanosensor based on LSPR for biological applications
Salbini, M.
Stomeo, T.
Ciraci, C.
Fiammengo, R.
Mangini, V
Toma, A.
Pisano, F.
Pisanello, F.
Verri, T.
Smith, D. R.
De Vittorio, M.
OPTICAL MATERIALS EXPRESS, 2020, 10 (05) : 1264 - 1272
[7] Automated tracking of label-free cells with enhanced recognition of whole tracks
Naim Al-Zaben
Anna Medyukhina
Stefanie Dietrich
Alessandra Marolda
Kerstin Hünniger
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[8] Automated tracking of label-free cells with enhanced recognition of whole tracks
Al-Zaben, Naim
Medyukhina, Anna
Dietrich, Stefanie
Marolda, Alessandra
Huenniger, Kerstin
Kurzai, Oliver
Figge, Marc Thilo
SCIENTIFIC REPORTS, 2019, 9 (1)
[9] Label-Free Live-Cell Imaging with Confocal Raman Microscopy
Klein, Katharina
Gigler, Alexander M.
Aschenbrenne, Thomas
Monetti, Roberto
Bunk, Wolfram
Jamitzky, Ferdinand
Morfill, Gregor
Stark, Robert W.
Schlegel, Juergen
BIOPHYSICAL JOURNAL, 2012, 102 (02) : 360 - 368
[10] Simultaneous label-free live imaging of cell nucleus and luminescent nanodiamonds
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Bela Varga
Elodie Middendorp
Orsolya Pall
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Frederic J. G. Cuisinier
Petr Cigler
Milos Nesladek
Csilla Gergely
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