Potential antiviral activity of rhamnocitrin against influenza virus H3N2 by inhibiting cGAS/STING pathway in vitro

被引：3
作者：
Chen, Zexing [1 ,2 ]
Wang, Wanqi [1 ,2 ]
Zeng, Kefeng [2 ]
Zhu, Jinyi [1 ,2 ]
Wang, Xinhua [1 ,2 ]
Huang, Wanyi [1 ,2 ]
机构：
[1] Guangzhou Med Univ, Guangzhou Inst Resp Hlth, Affiliated Hosp 1, State Key Lab Resp Dis,Natl Clin Res Ctr Resp Dis, Guangzhou 510180, Peoples R China
[2] Guangzhou Med Univ, Affiliated Hosp 1, Inst Integrat Tradit & Western Med, Guangzhou, Peoples R China
来源：
SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Rhamnocitrin; Influenza; cGAS; STING; Apoptosis; Anti-viral; ACTIVATION; PROPAGATION; APOPTOSIS; RESPONSES;
D O I：
10.1038/s41598-024-79788-z
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Influenza remains a serious issue for public health and it's urgent to discover more effected drugs against influenza virus. Rhamnocitrin, as a flavonoid, its effect on influenza virus infection remains poorly explored. In this study, rhamnocitrin showed antiviral effect and anti-apoptosis on influenza virus A/Aichi/2/1968 (H3N2) in MDCK cells and A549 cells. In addition, molecular docking revealed that rhamnocitrin have good binding activity with the target proteins cGAS and STING, molecular dynamic simulation and surface plasmon resonance showed that rhamnocitrin could form a stable complex with the above proteins. Moreover, the qPCR and western blot assays further verified that rhamnocitrin could reduce type I IFN and proinflammatory cytokines production by inhibiting the cGAS/STING pathway. Taken together, the results suggest that rhamnocitrin could be a potential anti-viral agent against influenza.
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页数：15
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