mRNA m6A detection

被引：0

作者：

Moshitch-Moshkovitz, Sharon ^{[1
,2
]}

Sevilla-Sharon, Michal ^{[2
]}

Ashwal-Fluss, Reut ^{[1
]}

Glick-Saar, Efrat ^{[1
,3
]}

Rechavi, Gideon ^{[1
,3
]}

Dominissini, Dan ^{[1
,2
]}

机构：

[1] Sheba Med Ctr, Wohl Inst Translat Med, Canc Res Ctr, Tel Hashomer, Israel

[2] Tel Aviv Univ, Tel Aviv, Israel

[3] Sheba Med Ctr, ARC Innovat Ctr, Tel Hashomer, Israel

来源：

NATURE REVIEWS METHODS PRIMERS | 2024年 / 4卷 / 01期

基金：

欧洲研究理事会; 以色列科学基金会;

关键词：

SINGLE-NUCLEOTIDE-RESOLUTION; MYELOID-LEUKEMIA; TRANSLATIONAL CONTROL; LOCAL TRANSLATION; MAPPING REVEALS; READ ALIGNMENT; NUCLEAR-RNA; METHYLATION; N-6-METHYLADENOSINE; CELL;

D O I：

10.1038/s43586-024-00365-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

N6-methyladenosine (m6A) is the most prevalent internal mRNA modification. Recent research has highlighted its role as a key regulator of gene expression, influencing cellular processes and determining cell fate. Advances in techniques for global mapping of m6A, the discovery of m6A demethylases that enhance its dynamic properties and the identification of reader proteins that interact with m6A have substantially propelled this field forward. This Primer outlines the available tools for detecting and mapping m6A, discusses the strengths and limitations of each method and offers guidance on selecting the most suitable approach. Identifying and detecting m6A lays the groundwork for functional studies that address important biological and medical questions.

引用

页数：20

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