Quantitative determination of leptin hormone using gold nanoparticle-based lateral flow assay

被引:0
作者
Zor, Erhan [1 ,2 ]
Alpaydin, Sabri [3 ]
Bingol, Haluk [1 ,4 ]
机构
[1] Necmettin Erbakan Univ, Sci & Technol Res & Applicat Ctr BITAM, Konya, Turkiye
[2] Necmettin Erbakan Univ, AK Educ Fac, Dept Sci Educ, Konya, Turkiye
[3] Necmettin Erbakan Univ, AK Educ Fac, Dept Chem Educ, Konya, Turkiye
[4] Necmettin Erbakan Univ, Fac Engn, Dept Basic Sci, Konya, Turkiye
关键词
Lateral flow assay; Colorimetric lateral flow reader; Leptin hormone; Gold nanoparticle; EXPRESSION; ADIPONECTIN; PROTEIN; URINE;
D O I
10.1007/s00604-024-06945-6
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A lateral flow assay (LFA) has been developed that can be used in point-of-care (PoC) use for the sensitive determination of leptin hormone. The limit of detection value was 0.158 ng/mL and the limit of quantification value was 0.479 ng/mL for leptin hormone. The stability studies showed that the assay response was equal to the initial value even after 6 months. LFA accuracy tests performed in commercial artificial serum samples were compared with the enzyme-linked immunosorbent assay (ELISA). The obtained recovery values between 95 and 110% show that the developed LFA can be used for quantitative determination of leptin hormone. As the result of the study, a fast and disposable assay was developed for the determination of leptin hormone, which can be performed with a small amount of sample in less than 15 min without the need for long-term analysis stages, qualified personnel, expensive equipment and devices.
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页数:9
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