Preparation of Antibacterial Nanofiber Nanocomposites Based on Bacterial Cellulose

This work introduces the creation of two innovative nanocomposite wound dressings: bacterial cellulose/g-C3N4 (GCN)/ginger and PVA/bacterial cellulose nanofibril (BCNF)/GCN/ginger, produced by immersion and electrospinning techniques. SEM analysis confirmed that the nanocomposites exhibit high porosity and uniform nanofiber structures, which enhance their structural integrity for wound healing applications. Antibacterial activity assays revealed significant inhibition of E. coli (88%) and S. aureus (92%) by the GCN/ginger composite, substantially surpassing the control samples. The MTT experiment demonstrated that both nanocomposites are non-cytotoxic, exhibiting cell survival rates of 95 +/- 4.73% for PVA/BCNF/GCN/ginger and 80 +/- 4.88% for BC/GCN/ginger. Additionally, in a scratch wound experiment, the nanocomposites exhibited significant wound healing efficacy, achieving decreases in wound area of 72.17 +/- 3.15% and 75.92 +/- 3.04% for PVA/BCNF/GCN/ginger and BC/GCN/ginger, respectively, in contrast to the control group's 85.93 +/- 3.44%. The results underscore the composites' exceptional antibacterial characteristics, cytocompatibility, and wound healing effectiveness, positioning them as attractive candidates for advanced wound dressing applications. However, this study is limited to in vitro experiments, and further in vivo and clinical studies are required to validate the safety, scalability, and therapeutic potential of these nanocomposites under physiological conditions.