Exogenous dsRNA triggers sequence-specific RNAi and fungal stress responses to control Magnaporthe oryzae in Brachypodium distachyon

被引：2

作者：

Zheng, Ying ^{[1
]}

Moorlach, Benjamin ^{[2
]}

Jakobs-Schoenwandt, Desiree ^{[2
]}

Patel, Anant ^{[2
]}

Pastacaldi, Chiara ^{[1
]}

Jacob, Stefan ^{[3
]}

Sede, Ana R. ^{[4
]}

Heinlein, Manfred ^{[4
]}

Poranen, Minna M. ^{[5
]}

Kogel, Karl-Heinz ^{[4
]}

Carmona, Maria Ladera ^{[1
]}

机构：

[1] Justus Liebig Univ Giessen, Inst Phytopathol, Res Ctr BioSyst Land Use & Nutr, Heinrich Buff Ring 26, D-35392 Giessen, Germany

[2] Bielefeld Univ Appl Sci, Bielefeld Inst Appl Mat Res, Fermentat & Formulat Biol & Chem, Interakt 1, D-33619 Bielefeld, Germany

[3] Inst Biotechnol & Drug Res, Hanns Dieter Husch Weg 17, D-55128 Mainz, Germany

[4] Univ Strasbourg, Inst Biol Mol Plantes, CNRS, 12 Rue Gen Zimmer, F-67084 Strasbourg, France

[5] Univ Helsinki, Fac Biol & Environm Sci, Mol & Integrat Biosci Res Programme, Helsinki, Finland

来源：

COMMUNICATIONS BIOLOGY | 2025年 / 8卷 / 01期

关键词：

INTERFERENCE; PROTECTION; GENE; PHOSPHORYLATION; PATHOGENICITY; KINASE;

D O I：

10.1038/s42003-025-07554-6

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In vertebrates and plants, dsRNA plays crucial roles as PAMP and as a mediator of RNAi. How higher fungi respond to dsRNA is not known. We demonstrate that Magnaporthe oryzae (Mo), a globally significant crop pathogen, internalizes dsRNA across a broad size range of 21 to about 3000 bp. Incubation of fungal conidia with 10 ng/mu L dsRNA, regardless of size or sequence, induced aberrant germ tube elongation, revealing a strong sequence-unspecific effect of dsRNA in this fungus. Accordingly, the synthetic dsRNA analogue poly(I:C) and dsRNA of various sizes and sequences elicited canonical fungal stress pathways, including nuclear accumulation of the stress marker mitogen-activated protein kinase Hog1p and production of ROS. Leaf application of dsRNA to the cereal model species Brachypodium distachyon suppressed the progression of leaf blast disease. Notably, the sequence-unspecific effect of dsRNA depends on higher doses, while pure sequence-specific effects were observed at low concentrations of dsRNA ( < 0.03 ng/<mu>L). The protective effects of dsRNA were further enhanced by maintaining a gap of at least seven days between dsRNA application and inoculation, and by stabilising the dsRNA in alginate-chitosan nanoparticles. Overall, our study opens up additional possibilities for the development and use of dsRNA pesticides in agriculture.

引用

页数：14