Different transcriptomic and metabolomic analysis of Saccharomyces cerevisiae BY4742 and CEN.PK2-1C strains

被引：0
作者：
Zhang, Meihong [1 ,2 ]
Zhao, Shujuan [1 ,2 ]
机构：
[1] Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, SATCM Key Lab New Resources & Qual Evaluat Chinese, MOE Key Lab Standardizat Chinese Med, Shanghai 201203, Peoples R China
[2] Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, Shanghai Key Lab Cpd Chinese Med, Shanghai 201203, Peoples R China
来源：
ARCHIVES OF MICROBIOLOGY | 2024年 / 206卷 / 12期
基金：
中国国家自然科学基金;
关键词：
Saccharomyces cerevisiae; Transcriptome; Metabolome; P-COUMARIC ACID; YEAST; GROWTH; DEHYDROGENASE; GENE;
D O I：
10.1007/s00203-024-04178-y
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
To establish efficient yeast cell factories, it is necessary to understand the transcriptional and metabolic changes among different yeasts. Saccharomyces cerevisiae BY4742 and CEN.PK2-1C strains are originated from different yeast strains and are commonly used as model organisms and chassis cells in molecular biology study and synthetic biology-based natural production. Metabolomic analysis showed that the BY4742 strain produced higher levels of phenylalanine, tyrosine than CEN.PK2-1C, while CEN.PK2-1C produced high levels of indoleacetaldehyde, indolepyruvate. Transcriptomic analysis showed that the two strains showed large differences in the glycolysis pathway and pyruvate metabolism pathway. CEN.PK2-1C had greater glycolysis flux than BY4742, whereas BY4742 has greater flux in the pathway of pyruvate metabolism to produce fumarate. These findings provide a basis knowledge of the metabolomic and transcriptomic differences between BY4742 and CEN.PK2-1C strains, and also provide preliminary information for strain selection for molecular biology study and synthetic biology-based natural product production.
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页数：10
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