Uncovering Hippo pathway-related biomarkers in acute myocardial infarction via scRNA-seq binding transcriptomics

被引：0

作者：

Li, Xingda ^{[1
,2
,3
,4
]}

He, Xueqi ^{[3
,4
]}

Zhang, Yu ^{[3
,4
]}

Hao, Xinyuan ^{[3
,4
]}

Xiong, Anqi ^{[6
]}

Huang, Jiayu ^{[6
]}

Jiang, Biying ^{[6
]}

Tong, Zaiyu ^{[6
]}

Huang, Haiyan ^{[5
]}

Yi, Lian ^{[5
]}

Chen, Wenjia ^{[6
]}

机构：

[1] Harbin Med Univ, Natl Key Lab Frigid Zone Cardiovasc Dis, State Prov Key Labs Biomed Pharmaceut China, Dept Pharmacol,Key Lab Cardiovasc Res,Minist Educ, Harbin 150086, Heilongjiang, Peoples R China

[2] Harbin Med Univ, Coll Pharm, Int Cooperat Base Major Cardiovasc Dis Cold Reg, Harbin 150086, Heilongjiang, Peoples R China

[3] Harbin Med Univ, Dept Pharm, Affiliated Hosp 2, Harbin 150086, Peoples R China

[4] Harbin Med Univ, Dept Pharmacol, Key Lab Cardiovasc Res, Minist Educ,Coll Pharm, Harbin 150086, Peoples R China

[5] Harbin Med Univ, Dept Neurol, Affiliated Hosp 1, Harbin 150001, Peoples R China

[6] Harbin Med Univ, Dept Cardiol, Affiliated Hosp 1, 23 YouZheng St, Harbin 150001, Heilongjiang, Peoples R China

来源：

SCIENTIFIC REPORTS | 2025年 / 15卷 / 01期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

Acute myocardial infarction; Hippo signaling pathway; Biomarkers; Immune infiltration; Endothelial cell; Macrophage; HEPATOCELLULAR-CARCINOMA; HEART-FAILURE; PROTEIN; EXPRESSION; SURVIVAL; NAD(+); KEGG;

D O I：

10.1038/s41598-025-94820-6

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This study investigated Hippo signaling pathway-related biomarkers in acute myocardial infarction (AMI). First, differentially expressed genes (DEGs) between AMI patients and controls were identified. Consensus clustering then classified AMI subtypes, followed by subtype-specific DEG screening. Candidate genes were derived from intersecting initial DEGs with subtype-associated DEGs. Three machine-learning algorithms prioritized five biomarkers (NAMPT, CXCL1, CREM, GIMAP6, and GIMAP7), validated through multi-dataset analyses and cellular expression profiling. qRT-PCR and Western blot confirmed differential expression patterns between AMI and controls across experimental models. Notably, NAMPT, CXCL1, and GIMAP6 exhibited cell-type-specific expression in endothelial cells and macrophages. We further predicted 179 potential therapeutic agents targeting these biomarkers. Niclosamide and eugenol were observed to mitigate hypoxia-induced injury in neonatal mouse ventricular cardiomyocytes. In vivo experiments demonstrated upregulated NAMPT/CXCL1 and downregulated GIMAP6/GIMAP7 in AMI myocardial tissues, with significant NAMPT protein elevation. These biomarkers show clinical diagnostic potential and provide mechanistic insights into AMI pathogenesis.

引用

页数：20