Identification of the Melon Fruit Fly Zeugodacus cucurbitae (Coquillett) (Diptera, Tephritidae) by Real-Time PCR with SYBR Green I Dye

被引：0
作者：
M. Yu. Arapova [1 ]
N. Yu. Oyun [2 ]
机构：
[1] Department of Entomology, Lomonosov Moscow State University, Moscow
[2] All-Russian Plant Quarantine Center, Bykovo, Moscow oblast
[3] Martsinovsky Institute of Medical Parasitology, Tropical and Vector-Borne Diseases, Sechenov First Moscow State Medical University, Moscow
[4] Centre for Strategic Planning and Management of Biomedical Health Risks of the Federal Medical and Biological Agency, Moscow
来源：
Entomological Review | 2024年 / 104卷 / 5期
基金：
俄罗斯科学基金会;
关键词：
COI gene; fruit flies; molecular identification; plant quarantine; real-time PCR; SYBR Green I;
D O I：
10.1134/S0013873824050026
中图分类号：
学科分类号：
摘要：
Abstract: The melon fly Zeugodacus cucurbitae (Coquillett) is a fruit pest mostly damaging plants of the family Cucurbitaceae and capable of causing significant economic losses. This species of Asian origin has spread to Africa, Australia, and Oceania. Preventing further dispersal of the melon fly in other regions requires a quick and reliable method for its identification, especially at the preimaginal stages that are most often found in fruits and are also more difficult to identify by morphological characters. During our study, species-specific primers for Z. cucurbitae mtDNA COI gene region were designed for the first time and used in real-time PCR with SYBR Green I dye to differentiate Z. cucurbitae from some other Tephritidae species occurring on the same host plants: Zeugodacus tau (Walker), Bactrocera dorsalis (Hendel), B. latifrons (Hendel), Anastrepha grandis (Macquart), Ceratitis capitata (Wiedemann), and two Dacus species. The results of sensitivity analysis of real-time PCR showed that the detection limit for DNA template was 1 ng/μL. The proposed method was successfully tested on both adults and third-instar larvae, so it can be used for research and in the future, for practical tasks of plant quarantine. © Pleiades Publishing, Ltd. 2024.
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页码：324 / 336
页数：12
相关论文
共 53 条
  • [1] Andrews K.J., Bester R., Manrakhan A., Maree H.J., A multiplex PCR assay for the identification of fruit flies (Diptera: Tephritidae) of economic importance in South Africa, Sci. Rep, 12, (2022)
  • [2] Arapova M., Kamayev I.O., Identification of some fruit fly larvae species (Insecta: Diptera: Tephritidae) on Cucurbitaceae fruits, Plant Health Quar, 1, (2022)
  • [3] Armstrong K.F., Cameron C.M., Frampton E.R., Fruit fly (Diptera: Tephritidae) species identification: a rapid molecular diagnostic technique for quarantine application, Bull. Entomol. Res, 87, (1997)
  • [4] Balmes V., Mouttet R., Development and validation of a simplified morphological identification key for larvae of tephritid species most commonly intercepted at import in Europe, EPPO Bull, 47, (2017)
  • [5] Barr N.B., Copeland R.S., De Meyer M., Masiga D., Kibogo H.G., Billah M.K., Osir E., Wharton R.A., McPheron B.A., Molecular diagnostics of economically important Ceratitis fruit fly species (Diptera: Tephritidae) in Africa using PCR and RFLP analyses, Bull. Entomol. Res, 96, (2006)
  • [6] Barr N.B., Islam M.S., De Meyer M., McPheron B.A., Molecular identification of Ceratitis capitata (Diptera: Tephritidae) using DNA sequences of the COI barcode region, Ann. Entomol. Soc. Am, 105, (2012)
  • [7] Barr N.B., Ruiz-Arce R., Farris R.E., Silva J.G., Lima K., Dutra V.S., Ronchi-Teles B., Kerr P.H., Norrbom A.L., Nolazco N., Thomas D.B., Identifying Anastrepha (Diptera
  • [8] Tephritidae) species using DNA barcodes, J. Econ. Entomol, 111, (2018)
  • [9] Bartolini I., Rivera J., Nolazco N., Olortegui A., Towards the implementation of a DNA barcode library for the identification of Peruvian species of Anastrepha (Diptera: Tephritidae), PLoS ONE, 15, 1, (2020)
  • [10] Benson D.A., Cavanaugh M., Clark K., Karsch-Mizrachi I., Ostell J., Pruitt K.D., Sayers E.W., GenBank, Nucleic Acids Res, 46, (2018)
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