Characterisation and hierarchy of the spermatogonial stem cell compartment in human spermatogenesis by spectral cytometry using a 16-colors panel

被引：0
|
作者：
Lapoujade, C. [1 ,2 ]
Blanco, M. [1 ,2 ]
Givelet, M. [1 ,2 ,3 ,4 ,5 ,6 ]
Gille, A. S. [1 ,2 ,3 ,4 ,5 ,6 ]
Allemand, I. [1 ,2 ]
Lenez, L. [3 ,4 ]
Thiounn, N. [6 ]
Roux, S. [6 ]
Wolf, J. P. [3 ,4 ,5 ,6 ]
Patrat, C. [3 ,4 ,5 ,6 ]
Riou, L. [1 ,2 ]
Barraud-Lange, V. [3 ,4 ,5 ,6 ]
Fouchet, P. [1 ,2 ]
机构：
[1] Univ Paris Saclay, IRCM IBFJ, Lab Cellules Souches Germinales, UMR Stabilite Genet Cellules Souches & Radiat,CEA, F-92265 Fontenay Aux Roses, France
[2] Univ Paris Cite, IRCM IBFJ, Lab Cellules Souches Germinales, UMR Stabilite Genet Cellules Souches & Radiat,CEA, F-92265 Fontenay Aux Roses, France
[3] Cochin Inst, Dept Genet & Cellular Plast, Team From Gametes To Birth, INSERM U1016, Paris, France
[4] Cochin Inst, Dept Metab & Endocrinol, Team From Gametes To Birth, INSERM U1016, Paris, France
[5] Univ Paris Cite, Paris, France
[6] Cochin Hosp, AP HP, Ctr Univ Paris Cite, Paris, France
来源：
CELLULAR AND MOLECULAR LIFE SCIENCES | 2024年 / 82卷 / 01期
关键词：
Spermatogonial stem cell; Human; Spermatogenesis; Spectral cytometry; TRANSPLANTATION; VISUALIZATION; TRANSCRIPTOME; ACTIVATION;
D O I：
10.1007/s00018-024-05496-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
About one in six couples experience fertility problems, and male infertility accounts for about half of these cases. Spermatogenesis originates from a small pool of spermatogonial stem cells (SSCs), which are of interest for the treatment of infertility but remain poorly characterised in humans. Using multiparametric spectral flow cytometric analysis with a 16-colours (16-C) panel of cell markers, we identify novel markers of SSCs and provide insights into unravelling and resolving the heterogeneity of the human spermatogonial cells. This 16-C panel of markers allowed the identification of a primitive SSCs state with the beta 2M-CD51/61-ITGA6+SSEA4+TSPAN33+THY1+CD9medEPCAMmedCD155+CD148+CD47highCD7high phenotype, with a profile close to the most primitive SSCs states 0 and SSC1-B previously defined by sc-RNAseq approach. The hierarchy of events in the spermatogonial stem cell and progenitor compartment of human spermatogenesis can be delineated. This highlights the importance of a multi-parametric and spectral cytometry approach. The in-depth characterisation of testicular cells should help to overcome the lack of stem cell knowledge, that hinders the understanding of the regenerative potential of SSCs, and is a critical parameter for the successful development of new SSCs-based cell therapies.
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页数：14
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