Type 2 vomeronasal receptor expression in the olfactory organ of African lungfish, Protopterus annectens

被引：1

作者：

Nakamuta, Shoko ^{[1
]}

Zhang, Zicong ^{[2
]}

Nikaido, Masato ^{[3
]}

Yokoyama, Takuya ^{[1
]}

Yamamoto, Yoshio ^{[1
]}

Nakamuta, Nobuaki ^{[1
]}

机构：

[1] Iwate Univ, Fac Agr, Lab Vet Anat, 3-18-8 Ueda, Morioka, Iwate 0208550, Japan

[2] Kyoto Univ, Inst Adv Study Human Biol, Yoshida Konoe Cho,Sakyo Ku, Kyoto, 6068501, Japan

[3] Tokyo Inst Technol, Sch Life Sci & Technol, 2-12-1 Ookayama,Meguro Ku, Tokyo 1528550, Japan

来源：

CELL AND TISSUE RESEARCH | 2024年 / 398卷 / 02期

关键词：

Evolution; In situ hybridization; Lungfish; RNA-seq; Vomeronasal organ; Vomeronasal receptors; PUTATIVE PHEROMONE RECEPTORS; EVOLUTIONARY DYNAMICS; MULTIGENE FAMILY; GENES; NEURONS; GLAND;

D O I：

10.1007/s00441-024-03918-2

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The olfactory organ of tetrapods, with few exceptions, comprises the main and accessory organs: olfactory epithelium (OE) and vomeronasal organ (VNO). Unlike tetrapods, teleost fish lack a VNO. However, lungfish, a type of sarcopterygian fish closely related to tetrapods, possesses a lamellar OE similar to the OE of teleosts and a recess epithelium (RecE) resembling the amphibian VNO. The RecE has been hypothesized as a primordial VNO. Olfactory receptors in tetrapods are distinctively expressed in the OE and VNO. For instance, type 2 vomeronasal receptors (V2Rs) in Xenopus are categorized into those exclusively expressed in the OE and those solely expressed in the VNO. It remains unclear whether V2Rs are differentially expressed between the lamellar OE and RecE in lungfish. This study investigated V2R expression in the lamellar OE and RecE of the African lungfish, Protopterus annectens. P. annectens V2Rs were categorized into three groups: those exclusively expressed in the lamellar OE, those exclusively expressed in the RecE, and those expressed in both the lamellar OE and RecE. V2Rs exclusively expressed in the RecE and those expressed in both the lamellar OE and RecE formed a distinct clade in the phylogenetic tree, whereas others were solely expressed in the lamellar OE. These findings suggest that lungfish V2R expression represents an intermediate stage toward complete segregation between V2Rs expressed in the OE and those expressed in the VNO.

引用

页码：79 / 91

页数：13