Investigation of functional characteristics of copper/copper oxide nanoparticles synthesized with Moringa oleifera and Musa sps. extracts: in-vitro and porcine study

The study analyzed the aqueous leaf extracts of Moringa oleifera and Musa sps. for phytochemical components, including flavonoids, sterols, saponins, tannins, and glycosides. The LC-MS analysis revealed gingerol, vicenin-2, caffeic acid, quercetin, and other compounds in the extracts. The synthesized MO- and MS- CuNPs (copper nanoparticles) exhibited plasmon resonance at 320 and 480 nm respectively, and Cu(II)-O stretching vibrations, manifested by their Fourier Transform Infrared (FT-IR) spectra. Thermogravimetric analysis showed that MO-/MS-CuNPs experienced a total weight loss of 80.2% and 68% respectively. Further, the EDS analysis revealed that MO-CuNPs have a copper content of 20%, while MS-CuNPs is 32%. The X-ray diffraction (XRD) patterns of the annealed MO and MS-CuNPs indicate the presence of both the cubic phase of copper (Cu) and the monoclinic tenorite phase of copper oxide (CuO). The percent DPPH scavenging activity of MO- and MS-CuNPs is 34.4 and 28.8, and by KMnO4 is 63.8 and 47.3% respectively. The behavior of CuNPs exhibited an asymmetrical response, with greater inhibition of Gram-negative bacteria compared to Gram-positive bacteria. CuNPs and medicinal plant-derived carbon dots were integrated into nanofibrous electrospun biopolymer scaffolds for wound care, demonstrating 100% wound healing in Yorkshire pigs full thickness excision wounds after 34 days.