Chromosome-scale genome assembly of Astragalus membranaceus using PacBio and Hi-C technologies

被引：3
作者：
Fan, Huijie [1 ]
Chai, Zhi [1 ]
Yang, Xukui [2 ]
Liu, Ake [3 ]
Sun, Haifeng [4 ]
Wu, Zhangyan [2 ]
Li, Qingshan [5 ,6 ]
Ma, Cungen [1 ]
Zhou, Ran [1 ]
机构：
[1] Shanxi Univ Chinese Med, Coll Basic Med Sci, Jinzhong 030619, Peoples R China
[2] BGI Genom, Shenzhen 518083, Peoples R China
[3] Changzhi Univ, Dept Life Sci, Changzhi 046011, Peoples R China
[4] Shanxi Univ, Coll Chem & Chem Engn, Taiyuan 030006, Peoples R China
[5] Shanxi Univ Chinese Med, Sch Chinese Mat Med, Jinzhong 030619, Peoples R China
[6] Shanxi Med Univ, Sch Pharmaceut Sci, Taiyuan 030001, Peoples R China
来源：
SCIENTIFIC DATA | 2024年 / 11卷 / 01期
关键词：
ALIGNMENT;
D O I：
10.1038/s41597-024-03852-6
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Astragalus membranaceus (Fisch.) Bge (AM) is a medicinal herb plant belonging to the Leguminosae family. In this study, we present a chromosome-scale genome assembly of AM, aiming to enhance the molecular biology and functional studies of Astragali Radix. The genome size of AM is about 1.43 Gb, with a contig N50 value of 1.67 Mb. A total of 98.16% of the assembly anchored to 9 pseudochromosomes using Hi-C technology. The assembly completeness was estimated to be 97.27% using BUSCO with the long terminal repeat assembly index (LAI) of 16.22 and quality value (QV) of 48.58. Additionally, the genome contained 67.98% repetitive sequences. Genome annotation predicted 29,914 protein-coding genes, including 73 genes involved in the flavonoid biosynthetic pathway and 2,048 transcription factors. The high-quality genome assembly and gene annotation resources will greatly facilitate future functional genomic studies in Leguminosae species.
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页数：11
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