Foliar application of 24-epibrassinolide enhances leaf nicotine content under low temperature conditions during the mature stage of flue-cured tobacco by regulating cold stress tolerance

被引：0

作者：

Chen, Haiyang ^{[1
,5
]}

Zhang, Shuaitao ^{[1
]}

Chang, Jianbo ^{[2
]}

Wei, Hongru ^{[1
]}

Li, Hongchen ^{[2
]}

Li, Chaoyang ^{[1
]}

Yang, Junjie ^{[2
]}

Song, Zhengxiong ^{[3
]}

Wang, Zhaojun ^{[1
]}

Lun, Jin ^{[6
]}

Zhang, Xuelin ^{[4
]}

Li, Lihua ^{[3
]}

Zhang, Xiaoquan ^{[1
]}

机构：

[1] Henan Agr Univ, Coll Tobacco Sci, Zhengzhou 450002, Peoples R China

[2] Henan Prov Tobacco Corp, Sanmenxia Branch, Sanmenxia 472000, Peoples R China

[3] Henan Prov Tobacco Corp, Luoyang Branch, Luoyang 471026, Peoples R China

[4] Henan Agr Univ, Coll Agron, State Key Lab Wheat & Maize Crop Sci, Zhengzhou 450046, Peoples R China

[5] Henan Agr Univ, Postdoctoral Stn Crop Sci, Zhengzhou 450046, Peoples R China

[6] China Tobacco Henan Ind Co LTD, Zhengzhou 450002, Peoples R China

来源：

BMC PLANT BIOLOGY | 2025年 / 25卷 / 01期

基金：

中国博士后科学基金;

关键词：

Nicotine; Tobacco; 24-epibrassinolide; EBR compound; Mature stage; TABACUM-L; NITROGEN-METABOLISM; CELL ELONGATION; TOTAL ALKALOIDS; ARABIDOPSIS; CYTOCHROME-P450; GENES; MAIZE; YIELD; INHERITANCE;

D O I：

10.1186/s12870-025-06080-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

BackgroundLow temperatures disrupt nitrogen metabolism in tobacco, resulting in lower nicotine content in the leaves. 24-epibrassinolide (EBR) is a widely used plant growth regulator known for its roles in enhancing cold tolerance and nitrogen metabolism. Nevertheless, it remains unclear whether EBR enhances leaf nicotine content under low temperature conditions during the mature stage of flue-cured tobacco.ResultsTo investigate the effects of EBR on leaf nicotine content under low temperature conditions during the mature stage of 'Yunyan 87' flue-cured tobacco, four treatments (foliar spraying of 0, 0.1, 0.2 and 0.4 mg<middle dot>L- 1 EBR solutions) were performed by using a single-factor randomized complete block design. The result showed that foliar spraying of different concentrations of EBR notably improve the agronomic and economic traits of flue-cured tobacco to varying degrees, as well as increase the total nitrogen and nicotine content in the tobacco leaves. 0.2 mg<middle dot>L- 1 EBR treatment showed better results, with nicotine content in the middle and upper leaves after curing increasing by 11.11% and 19.90%, respectively, compared to CK. Compared to the single EBR, foliar spraying of EBR compound containing alpha-Cyclodextrin and Tween 80 prolongs the effect of EBR, promotes the growth and development of tobacco plants. Combining EBR with CaCl2 and ZnSO4<middle dot>7H2O significantly enhances the cold resistance of tobacco plants. Furthermore, combining EBR with higher concentrations of KH2PO4 is more effective in promoting the maturation and yellowing of the upper leaves than those with lower concentrations.ConclusionsThis study provides new insights that foliar application of EBR enhances leaf nicotine content under low temperature conditions during the mature stage of flue-cured tobacco by regulating cold stress tolerance. The integration of EBR with alpha-Cyclodextrin, Tween 80, CaCl2, ZnSO4<middle dot>7H2O and KH2PO4 showcases a novel approach to extending the effectiveness of plant growth regulators and improving agricultural sustainability. Furthermore, these findings may be applicable to other cold-sensitive crops, offering broader benefits for improving resilience and productivity under low temperatures. However, the research focuses on two growth cycles, without investigating the long-term impact of EBR on soil health, crop sustainability, and ecosystem. And further research is needed to elucidate the molecular mechanisms of EBR on enhancing leaf nicotine content.Clinical trial numberNot applicable.

引用

页数：16