A novel antifungal chitinase from Chaetomium globosum: column-free purification and characterization

被引:0
作者
Jiang, Cheng [1 ,4 ,5 ]
Li, Luli [2 ]
Li, Jialu [1 ,3 ]
机构
[1] Huainan Normal Univ, Sch Biol Engn, Huainan, Peoples R China
[2] Huainan 2 Sr High Sch, Huainan, Peoples R China
[3] LanZhou Inst Biol Prod, Lanzhou, Peoples R China
[4] Huainan Normal Univ, Sch Biol Engn, Huainan, Peoples R China
[5] Huainan Normal Univ, Inst Digital Ecol & Hlth, Huainan, Peoples R China
关键词
Chitinase; Chaetomium globosum; Column-free purification; Hydrolysis; Antifungal; ENTOMOPATHOGENIC FUNGUS; CLONING; EXOCHITINASE; RESOURCE; GENE;
D O I
10.1007/s00203-025-04295-2
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A new chitinase gene, cloned from the biocontrol Chaetomium globosum W7, was designated Cgchi18. Recombinant protein Cgchi18 with 535 amino acids was expressed in Escherichia coli, and purified by means of a column-free purification method relying on split intein, achieving a 12.39-fold purification and a 15.61% recovery yield. The maximum activity of this approximately 60-kDa protein was observed at 45 degrees C and pH 5.0. Cgchi18 was activated by Mg2+ and Ba2+, but inhibited by Mn2+, Co2+, Cu2+, Zn2+, Ag+ and Hg2+. Cgchi18 showed high substrate specificity, only hydrolyzing beta-1,4-glycoside bond in chitin and its derivatives, to liberate disaccharides or trisaccharides. For the degradation of colloidal chitin under optimal conditions, Vmax and Km of Cgchi18 were calculated as 8.05 mu mol/min/mg and 3.18 mg/mL, respectively. Additionally, it exhibited antifungal activity and could have a degrading effect on the spread of hyphae of pathogenic fungi. In conclusion, the chitinase Cgchi18 identified from C. globosum has potential for industrial and agricultural applications.
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页数:12
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