Chromosome-level and haplotype-resolved genome assembly of Bougainvillea glabra

被引:0
作者
Lan, Lan [1 ,2 ,3 ]
Li, Haiyan [1 ,4 ]
Xu, Shisong [1 ,4 ]
Xu, Yueting [1 ,5 ]
Leng, Qingyun [1 ,4 ]
Zhang, Linbi [6 ]
Wu, Linqiao [7 ]
Yin, Junmei [1 ,4 ,8 ]
Wu, Zhiqiang [2 ]
Niu, Junhai [1 ,4 ]
机构
[1] Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Natl Key Lab Trop Crop Breeding, Key Lab Gene Resources & Germplasm Enhancement Sou, Haikou, Peoples R China
[2] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Guangdong Lab Lingnan Modern Agr, Genome Anal Lab,Minist Agr & Rural Affairs,Shenzhe, Shenzhen, Peoples R China
[3] Murdoch Univ, Coll Sci Hlth Engn & Educ, State Agr Biotechnol Ctr SABC, Perth, WA 6150, Australia
[4] Engn Technol Res Ctr Trop Ornamental Plant Germpla, Danzhou, Peoples R China
[5] Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China
[6] Hainan Daxin Landscape Co Ltd, Haikou, Peoples R China
[7] Hainan Big Lake Bridge Technol Co Ltd, Haikou, Peoples R China
[8] Chinese Acad Trop Agr Sci, Sanya Res Inst, Sanya, Peoples R China
关键词
HI-C; ALIGNMENT;
D O I
10.1038/s41597-024-04333-6
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Bougainvillea glabra Choisy, a common perennial shrub or climbing vine in tropical and subtropical areas, has significant ornamental and ecological value due to its showy floriferous blooms and adaptability to multiple environmental stresses. In this study, based on PacBio and Hi-C sequencing data, we presented the haplotype-resolved, and chromosome-level assembled genomes for B. glabra (2n = 34). We obtained two haplotypes with genome size of 2,855,692,602 bp and 2,889,511,499 bp, N50 of 165 Mbp and 167 Mbp, and both anchored to 17 chromosomes with anchor ratio of 97.07% and 97.55%, respectively. Through the BUSCO evaluation process, the two assembled haplotypes both exhibited the high score of 96%. By combining the high-confidence different evidence of genome structure annotation pipeline we annotated each haplotype containing similar to 33,000 predicted genes, of which 86% were functionally annotated by different databases. These high-quality haplotypes assembly and annotation results will serve as a great genomic resource to explore the ornamental traits and environmental adaptability and accelerate improvement in future.
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页数:7
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