Diffractive Optical Elements for Three-Dimensional Nanoscopy Using Rotating Light Fields

被引：0
作者：
Prokopova, D.V. [1 ]
Eremchev, I. Yu. [2 ,3 ]
Losevsky, N.N. [1 ]
Belousov, D.A. [4 ]
Golubtsov, S.K. [4 ]
Kotova, S.P. [1 ]
Naumov, A.V. [2 ,3 ]
机构：
[1] Lebedev Physical Institute, Russian Academy of Sciences, Samara
[2] Institute of Spectroscopy, Russian Academy of Sciences, Moscow
[3] Troitsk Branch, Lebedev Physical Institute, Russian Academy of Sciences, Moscow
[4] Institute of Automation and Electrometry, Siberian Branch, Russian Academy of Sciences, Novosibirsk
来源：
Bulletin of the Russian Academy of Sciences: Physics | 2024年 / 88卷 / 12期
关键词：
diffractive optical elements; spiral light beams; three-dimensional nanoscopy;
D O I：
10.1134/S1062873824708390
中图分类号：
学科分类号：
摘要：
Abstract: The authors study diffractive optical elements manufactured via contact printing on a dichromated gelatin and direct laser writing on photoresist. The elements modify the point-spread function for three-dimensional super resolution fluorescence microscopy. It is shown that both elements produce two-lobed rotating light fields that can be used for three-dimensional nanoscopy. Results from the subdiffractive localization of fluorescent labels are presented, and the accuracy of coordinate reconstruction is determined. © Pleiades Publishing, Ltd. 2024.
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页码：1875 / 1880
页数：5
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