Source-specific health effects of internally exposed organics in urban PM2.5 based on human serum albumin adductome analysis

被引：0

作者：

Zeng, Yaling ^{[1
,2
]}

Yang, Xin ^{[1
,2
]}

Zhang, Antai ^{[1
,2
]}

Yuan, Xin ^{[1
,2
]}

Zhai, Jinghao ^{[1
,2
]}

Xing, Chunbo ^{[1
,2
]}

Cai, Baohua ^{[1
,2
]}

Shi, Shao ^{[1
,2
]}

Zhang, Yin ^{[1
,2
]}

Zhang, Yujie ^{[1
,2
]}

机构：

[1] Shenzhen Key Laboratory of Precision Measurement and Early Warning Technology for Urban Environmental Health Risks, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen

[2] Guangdong Provincial Observation and Research Station for Coastal Atmosphere and Climate of the Greater Bay Area, Shenzhen

来源：

Science of the Total Environment | 2024年 / 955卷

基金：

中国国家自然科学基金;

关键词：

Human serum albumin; Internal exposure; Non-covalent adductome; Non-target analysis; PM[!sub]2.5[!/sub;

D O I：

10.1016/j.scitotenv.2024.176958

中图分类号：

学科分类号：

摘要：

Once inhaled, organic compounds in ambient PM2.5 permeate the bloodstream, resulting in internal exposure. The intricate composition of these internalized organic molecules complicates the processes of source attribution and toxicity assessment. A systematic framework to assess the health impacts of water-soluble organic molecules (WSOMs) originating from diverse sources is still undeveloped. This study aims to comprehensively analyze the source-specific health effects of internalized organics in urban PM2.5 through human serum albumin (HSA) non-covalent adductomes with WSOMs. Using high-resolution mass spectrometry, surface plasmon resonance, and machine learning, we mapped HSA-WSOM interactions, uncovering WSOM's potential internal exposure through its HSA adductome. The study identified eight distinct sources of internalized WSOMs, primarily from biogenic emissions, gasoline exhaust, and biomass combustion. Notably, WSOMs from these sources exhibited a predominant interaction with HSA residues ARG257, LEU238, and TRP150, substantially altering the functional dynamics of fatty acid binding site two and the hydrophobic cavity via hydrogen bonding and hydrophobic interactions. The primary health impacts of internalized WSOMs were identified as neurotoxicity and respiratory toxicity. WSOMs originating from biogenic sources and ocean emissions were mainly responsible for neurotoxic effects, whereas those from biomass burning and gasoline exhaust predominantly caused respiratory toxicity. Using the HSA adductome framework, our study identifies source-specific profiles and health effects of internally exposed WSOMs in urban PM2.5, emphasizing the importance of targeted mitigation strategies. © 2024

引用

共 25 条

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