Immune infiltration related circular RNA, circGLIS2, facilitated progression of endometriosis through miR-4731-5p/IL-1β axis

被引：1
作者：
Yin, Meichen [1 ,2 ]
Wang, Jianzhang [1 ,3 ]
Zhang, Xinmei [1 ,3 ]
机构：
[1] Zhejiang Univ, Womens Hosp, Dept Gynecol, Sch Med, Hangzhou 310006, Peoples R China
[2] Peking Univ, Peoples Hosp, Dept Obstet & Gynecol, Beijing 100044, Peoples R China
[3] Zhejiang Univ, Womens Hosp, Zhejiang Prov Key Lab Precis Diag & Therapy Major, Sch Med, Hangzhou 310006, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2024年 / 281卷
基金：
中国国家自然科学基金;
关键词：
circGLIS2; miR-4731-5p; IL-1; beta; Immune infiltration; Endometriosis; IL-1; FAMILY; PATHOGENESIS; INFLAMMATION; CERNA; WOMEN; MODEL;
D O I：
10.1016/j.ijbiomac.2024.136318
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Aberrantly expressed circRNAs affects various anti-tumour immune responses and immune cell regulation. However, the exact function of circGLIS2 on the pathogenesis of EMs remains unclear. In this study, we found that circGLIS2 was upregulated in EMs tissues and intimately related to clinicopathologic characteristics of EMs patients. Functionally, IHC and mouse model of EMs proved that circGLIS2 recruited immune cells infiltrated into ectopic endometrial microenvironment. RNA-seq, ELISA, RT-qPCR, and western blot results indicated that circGLIS2 influenced immune infiltration by regulating IL-1 beta. Besides, expression of circGLIS2 and IL-1 beta was also closely correlated in 284 human endometrium tissues. Mechanistically, RNA sequencing and intermolecular interaction prediction established "circGLIS2-miR-4731-5p-IL-1 beta" network. RNA pull down, RIP, dual luciferase reporter assay and expression regulation analysis confirmed the interaction among "circGLIS2-miR-4731-5p-IL1 beta" axis. In summary, our findings demonstrated that circGLIS2 facilitated EMs progression by increasing immune cell infiltration via miR-4731-5p/IL-1 beta axis.
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页数：13
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