TssL2 of T6SS2 is required for mobility, biofilm formation, wrinkly phenotype formation, and virulence of Vibrio parahaemolyticus SH112

被引:0
作者
Bai, Xue-rui [1 ,2 ]
Liu, Peng-xuan [1 ]
Wang, Wen-chao [1 ]
Jin, Ying-hong [3 ]
Wang, Quan [1 ]
Qi, Yu [1 ]
Zhang, Xiao-yun [1 ]
Sun, Wei-dong [4 ]
Fang, Wei-huan [5 ,6 ]
Han, Xian-gan [1 ,7 ]
Jiang, Wei [1 ]
机构
[1] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China
[2] Shanghai Vocat Coll Agr & Forestry, Dept Anim Sci & Technol, Shanghai 201699, Peoples R China
[3] Xinjiang Acad Anim Sci, Inst Vet Med, Urumqi 830013, Peoples R China
[4] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China
[5] Zhejiang Univ, Inst Prevent Vet Med, Hangzhou 310058, Peoples R China
[6] Zhejiang Univ, Zhejiang Prov Key Lab Prevent Vet Med, Hangzhou 310058, Peoples R China
[7] Longyan Univ, Engn Res Ctr Prevent & Control Anim Original Zoono, Longyan 364012, Peoples R China
基金
中国国家自然科学基金;
关键词
Vibrio parahaemolyticus; T6SS2; Motility; Biofilm; Wrinkly phenotype; Cytotoxicity; VI SECRETION SYSTEM; ABC TRANSPORTER; EXPRESSION; MOTILITY; ADHESION; GENES; TRANSCRIPTION; PATHOGENESIS; ACTIVATION; BINDING;
D O I
10.1007/s00253-024-13351-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Type VI secretion system 2 (T6SS2) of Vibrio parahaemolyticus is required for cell adhesion and autophagy in macrophages; however, other phenotypes conferred by this T6SS have not been thoroughly investigated. We deleted TssL2, a key component of T6SS2 assembly, to explore the role of the T6SS2 in environmental adaptation and virulence. TssL2 deletion reduced Hcp2 secretion, suggesting that TssL2 played an important role in activity of functional T6SS2. We found that TssL2 was necessary for cell aggregation, wrinkly phenotype formation, and participates in motility and biofilm formation by regulating related genes, suggesting that TssL2 was essential for V. parahaemolyticus to adapt changing environments. In addition, this study demonstrated TssL2 significantly affected adhesion, cytotoxicity, bacterial colonization ability, and mortality in mice, even the levels of the proinflammatory cytokines IL-6 and IL-8, suggesting that TssL2 was involved in bacterial virulence and immunity. Proteome analysis revealed that TssL2 significantly affected the expression of 163 proteins related to ABC transporter systems, flagellar assembly, biofilm formation, and multiple microbial metabolism pathways, some of which supported the effect of TssL2 on the different phenotypes of V. parahaemolyticus. Among them, the decreased expression of the T3SS1 and T2SS proteins was confirmed by the results of gene transcription, which may be the main reason for the decrease in cytotoxicity. Altogether, these findings further our understanding of T6SS2 components on environmental adaption and virulence during bacterial infection.
引用
收藏
页数:21
相关论文
共 79 条
  • [1] Genetic Relationship, Virulence Factors, Drug Resistance Profile and Biofilm Formation Ability of Vibrio parahaemolyticus Isolated From Mussel
    Ashrafudoulia, Md
    Miza, Md Furkanur Rahaman
    Park, Heedae
    Byun, Kye-Hwan
    Lee, Nari
    Park, Si Hong
    Ha, Sang-Do
    [J]. FRONTIERS IN MICROBIOLOGY, 2019, 10
  • [2] &ITVibrio cholerae&IT O1 secretes an extracellular matrix in response to antibody-mediated agglutination
    Baranova, Danielle E.
    Levinson, Kara J.
    Mantis, Nicholas J.
    [J]. PLOS ONE, 2018, 13 (01):
  • [3] DotU and VgrG, Core Components of Type VI Secretion Systems, Are Essential for Francisella LVS Pathogenicity
    Broms, Jeanette E.
    Meyer, Lena
    Lavander, Moa
    Larsson, Par
    Sjostedt, Anders
    [J]. PLOS ONE, 2012, 7 (04):
  • [4] An ABC transporter and an outer membrane lipoprotein participate in posttranslational activation of type VI secretion in Pseudomonas aeruginosa
    Casabona, Maria G.
    Silverman, Julie M.
    Sall, Khady M.
    Boyer, Frederic
    Coute, Yohann
    Poirel, Jessica
    Grunwald, Didier
    Mougous, Joseph D.
    Elsen, Sylvie
    Attree, Ina
    [J]. ENVIRONMENTAL MICROBIOLOGY, 2013, 15 (02) : 471 - 486
  • [5] A PhoPQ-Regulated ABC Transporter System Exports Tetracycline in Pseudomonas aeruginosa
    Chen, Lin
    Duan, Kangmin
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2016, 60 (05) : 3016 - 3024
  • [6] Structure and Activity of the Type VI Secretion System
    Cherrak, Yassine
    Flaugnatti, Nicolas
    Durand, Eric
    Journet, Laure
    Cascales, Eric
    [J]. MICROBIOLOGY SPECTRUM, 2019, 7 (04):
  • [7] The Type VI secretion system: a versatile bacterial weapon
    Coulthurst, Sarah
    [J]. MICROBIOLOGY-SGM, 2019, 165 (05): : 503 - 515
  • [8] Isolation and structural characterization of Coryxin, a novel cyclic lipopeptide from Corynebacterium xerosis NS5 having emulsifying and anti-biofilm activity
    Dalili, Dina
    Amini, Mohsen
    Faramarzi, Mohammad Ali
    Fazeli, Mohammad Reza
    Khoshayand, Mohammad Reza
    Samadi, Nasrin
    [J]. COLLOIDS AND SURFACES B-BIOINTERFACES, 2015, 135 : 425 - 432
  • [9] Characterization of IcmF of the type VI secretion system in an avian pathogenic Escherichia coli (APEC) strain
    de Pace, Fernanda
    de Paiva, Jacqueline Boldrin
    Nakazato, Gerson
    Lancellotti, Marcelo
    Sircili, Marcelo Palma
    Stehling, Eliana Guedes
    da Silveira, Wanderley Dias
    Sperandio, Vanessa
    [J]. MICROBIOLOGY-SGM, 2011, 157 : 2954 - 2962
  • [10] Type 1 fimbriae-mediated collective protection against type 6 secretion system attacks
    Dessartine, Margot Marie
    Kosta, Artemis
    Doan, Thierry
    Cascales, Eric
    Cote, Jean-Philippe
    [J]. MBIO, 2024, 15 (04):