Dead Sea water as a sustainable source for the production of microbial bioplastics polyhydroxyalkanoates by halophiles

被引:0
作者
Alsafadi, Diya [1 ]
Almashaqbeh, Othman [2 ]
Oqdeh, Saba [1 ]
Mansour, Aya [1 ]
机构
[1] Royal Sci Soc, Res Ind Ctr, Biocatalysis & Biosynth Res Unit, Amman 11941, Jordan
[2] Royal Sci Soc, Emerging Pollutants Res Unit, Amman, Jordan
来源
BIORESOURCE TECHNOLOGY REPORTS | 2024年 / 28卷
关键词
Polyhydroxyalkanoates; Seawater; Biodegradable plastic; Haloferax mediterranei; Fed-batch fermentation; Food and agro-industrial waste; POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE); WASTE;
D O I
10.1016/j.biteb.2024.101989
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The Dead Sea is a unique salt-saturated water body. This work utilized the Dead Sea water (DSW) as a medium instead of fresh water for the biosynthesis of polyhydroxyalkanoates (PHAs) by Haloferax mediterranei. The intracellular content of PHAs reached a maximum (6.35 %) at 40 % DSW. An artificial DSW media that mimics the DSW composition was prepared and used for PHAs production by H. mediterranei. The cell dry mass (CDM) and PHAs comcentration (32.61 g L- 1 and 4.56 g L- 1, respectively) were significantly higher than the results obtained from the reported H. mediterranei highly saline medium. The PHAs production was scaled-up to fedbatch utilizing no-cost date fruit waste and resulted in CDM and PHA concentration of 46.89 g L- 1 and 12.77 g L- 1, respectively under non-sterile conditions. H. mediterranei accumulated poly-3-(hydroxybutyrate-co-9 %-hydroxyvalerate) with molar mass 916.0 kDa and melting point at 143.2 degrees C. These results indicate the production of a high-quality biopolymer.
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页数:7
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