Rational design of a ratiometric fluorescent probe for selective visualizing peroxynitrite in mitochondria and evaluation of organ injury

被引：0
作者：
Liu, Lingyan [1 ]
Li, Ruohan [2 ]
Liu, Feiyang [2 ]
Wei, Peng [1 ]
Yi, Tao [1 ,2 ]
机构：
[1] State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Chemistry and Chemical Engineering, Donghua University, Shanghai
[2] Department of Chemistry, Fudan University, 2005 Songhu Road, Shanghai
来源：
Sensors and Actuators B: Chemical | 2024年 / 419卷
基金：
中国国家自然科学基金;
关键词：
Intramolecular charge transfer; Mitochondria; Organ injury; Peroxynitrite; Ratiometric fluorescence;
D O I：
10.1016/j.snb.2024.136421
中图分类号：
学科分类号：
摘要：
Abnormal peroxynitrite (ONOO−) expression is closely associated with the development of various pathological processes. In this study, we devised a series of fluorescent probes, namely DHU-DCM-1, DHU-DCM-2, and DHU-DCM-3, tailored specifically for monitoring ONOO− through rationally molecular design. By modulating the electron-donating site within the fluorophore structure, the optimal probe DHU-DCM-3 exhibited excellent ratiometric detecting performance. DHU-DCM-3 displayed high selectivity and sensitivity towards ONOO−, effectively distinguishing it from other reactive oxygen species. Notably, DHU-DCM-3 was capable of labeling mitochondrial ONOO−, producing an enhanced fluorescence ratio (F656/F550), thereby allowing for the visualization of ONOO− fluctuations in both exogenous and endogenous contexts within living cells. The utility of DHU-DCM-3 was further demonstrated in various applications. For instance, through detecting elevated levels of ONOO−, senile C. elegans was successfully identified by utilizing DHU-DCM-3. Moreover, relying on its ratiometric fluorescence, DHU-DCM-3 was proved to be valuable in accurately diagnosing liver injury in mice with varying degrees of severity. Overall, we have constructed a ratiometric fluorescent probe for detecting and imaging ONOO− in both subcellular organelle and in vivo, highlighting its potential significance in various biomedical applications. © 2024 Elsevier B.V.
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