: a new class of fluorescent cellular and tissue membrane probe

被引：4
作者：
Anand, Saurabh [1 ]
Bhoge, Preeti Ravindra [1 ]
Raigawali, Rakesh [1 ]
Saladi, Srinivas Vinod [2 ]
Kikkeri, Raghavendra [1 ,3 ]
机构：
[1] Indian Inst Sci Educ & Res, Dept Chem, Pune 411008, India
[2] Univ Toledo, Coll Med & Life Sci, Dept Cell & Canc Biol, Toledo, OH 43614 USA
[3] Jackson State Univ, Dept CPAS, Jackson, MS 39217 USA
来源：
CHEMICAL SCIENCE | 2024年 / 15卷 / 47期
关键词：
BASEMENT-MEMBRANE; PLASMA-MEMBRANES; LIPID ORDER;
D O I：
10.1039/d4sc06225f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The development of long-lasting plasma membrane (PM) and basement membrane (BM) probes is in high demand to advance our understanding of membrane dynamics during differentiation and disease conditions. Herein, we report that the microheterogeneity of heparan sulfate (HS) on fluorescent neo-proteoglycans backbone offers a facile platform for designing membrane probes. Confocal live-cell imaging studies of cancer and normal cell lines with a panel of Cy5 fluorescently tagged neo-proteoglycans confirmed that highly sulfated HS ligands with an l-iduronic acid component (PG@ID-6) induce a prolonged and brighter expression on the PM compared to low-sulfated and uronic acid counterparts. Mono- and multi-photon microscopic imaging of tissue sections with NeoMProbe (PG@ID-6) allowed mapping BM and demonstrated staining efficacy equivalent to antibodies against the BM components. Finally, in vivo, whole-body imaging of NeoMProbe and subsequent tissue section imaging confirmed versatile and efficient membrane mapping by the probe. Overall, NeoMProbe offers a novel toolkit for cell biology and tissue biomembrane imaging.
引用
收藏
页码：19962 / 19969
页数：8
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[1]   Molecular recognition and proteoglycan mimic arrangement: modulating cisplatin toxicity [J].
Anand, Saurabh ;
Mardhekar, Sandhya ;
Bhoge, Preeti Ravindra ;
Mishra, Sandeep Kumar ;
Kikkeri, Raghavendra .
CHEMICAL COMMUNICATIONS, 2024, 60 (33) :4495-4498
[2]   Fluorogenic Dimers as Bright Switchable Probes for Enhanced Super-Resolution Imaging of Cell Membranes [J].
Aparin, Ilya O. ;
Yan, Rui ;
Pelletier, Remi ;
Choi, Alexander A. ;
Danylchuk, Dmytro I. ;
Xu, Ke ;
Klymchenko, Andrey S. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2022, 144 (39) :18043-18053
[3]   Synthesis of a Systematic 64-Membered Heparan Sulfate Tetrasaccharide Library [J].
Baryal, Kedar N. ;
Ramadan, Sherif ;
Su, Guowei ;
Huo, Changxin ;
Zhao, Yuetao ;
Liu, Jian ;
Hsieh-Wilson, Linda C. ;
Huang, Xuefei .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2023, 62 (01)
[4]   Synergestic interplay of uronic acid and sulfation composition of heparan sulfate on molecular recognition to activity [J].
Bhoge, Preeti Ravindra ;
Raigawali, Rakesh ;
Mardhekar, Sandhya ;
Anand, Saurabh ;
Kikkeri, Raghavendra .
CARBOHYDRATE RESEARCH, 2023, 532
[5]  
Cabrera M., 2012, IntraVital, V1, P107, DOI DOI 10.4161/INTV.23423
[6]   Synthetic Heparanase Inhibitors Can Prevent Herpes Simplex Viral Spread [J].
Chopra, Pradeep ;
Yadavalli, Tejabhiram ;
Palmieri, Francesco ;
Jongkees, Seino A. K. ;
Unione, Luca ;
Shukla, Deepak ;
Boons, Geert-Jan .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2023, 62 (41)
[7]   Advanced functional fluorescent probes for cell plasma membranes [J].
Collot, Mayeul ;
Pfister, Sonia ;
Klymchenko, Andrey S. .
CURRENT OPINION IN CHEMICAL BIOLOGY, 2022, 69
[8]   Molecular Tuning of Styryl Dyes Leads to Versatile and Efficient Plasma Membrane Probes for Cell and Tissue Imaging [J].
Collot, Mayeul ;
Boutant, Emmanuel ;
Fam, Kyong Tkhe ;
Danglot, Lydia ;
Klymchenko, Andrey S. .
BIOCONJUGATE CHEMISTRY, 2020, 31 (03) :875-883
[9]   MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience [J].
Collot, Mayeul ;
Ashokkumar, Pichandi ;
Anton, Halina ;
Boutant, Emmanuel ;
Faklaris, Orestis ;
Galli, Thierry ;
Mely, Yves ;
Danglot, Lydia ;
Klymchenko, Andrey S. .
CELL CHEMICAL BIOLOGY, 2019, 26 (04) :600-+
[10]   Bright fluorogenic squaraines with tuned cell entry for selective imaging of plasma membrane vs. endoplasmic reticulum [J].
Collot, Mayeul ;
Kreder, Remy ;
Tatarets, Anatoliy L. ;
Patsenker, Leonid D. ;
Mely, Yves ;
Klymchenko, Andrey S. .
CHEMICAL COMMUNICATIONS, 2015, 51 (96) :17136-17139
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