Nanosilver-Enhanced Far-Field Fluorescence Fluctuations for Super-Resolution Microscopy

被引：0

作者：

Qin, Guangyong ^{[1
,2
]}

Guan, Xin ^{[1
,2
]}

Mao, Jian ^{[1
,2
]}

Feng, Zhenzhen ^{[2
,3
]}

Yang, Wenxuan ^{[1
,2
]}

Wang, Shenming ^{[1
,2
]}

Zhen, Zheng ^{[1
,2
]}

Miao, Xintong ^{[1
,2
]}

Cheng, Yifeng ^{[1
,2
]}

Wang, Zhirui ^{[1
,2
]}

Wang, Xiaojuan ^{[1
,2
]}

Huang, Fang ^{[1
,2
]}

He, Hua ^{[1
,2
]}

机构：

[1] China Univ Petr East China, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China

[2] China Univ Petr East China, Coll Chem & Chem Engn, Qingdao 266580, Peoples R China

[3] Tech Ctr Qingdao Customs Dist, Qingdao 266500, Peoples R China

来源：

NANO LETTERS | 2024年 / 24卷 / 47期

基金：

中国国家自然科学基金;

关键词：

Super-resolution; Enhancement; Fluorescencefluctuations; Nanosilver; Far-field; PROTEIN;

D O I：

10.1021/acs.nanolett.4c05155

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Fluctuation-based super-resolution microscopy enhances image resolution using signal fluctuations, yet the inherent fluctuations of fluorophores limit its spatiotemporal resolution. In this work, we reveal a far-field enhancement (FFE) effect via a nanosilver film that significantly boosts fluorescence fluctuations of fluorophores positioned up to 10 mu m away. The FFE effect arises from the interference of scattered light from the nanosilver film and photothermal-induced refractive index changes in the imaging medium, which create periodic auxiliary illumination on the sample. This phenomenon enabled the development of far-field enhanced super-resolution microscopy (FFE-SRM), a technique compatible with commonly used fluorophores. FFE-SRM improves temporal resolution up to 10-fold and enhances spatial resolution by about 2-fold over various SRM methods, including stochastic optical fluctuation imaging, super-resolution radial fluctuation, mean-shift super-resolution, and direct stochastic optical reconstruction microscopy. We demonstrated the potential of FFE-SRM by revealing mitochondrial dynamics in live-cell imaging, advancing super-resolution imaging, and cellular process exploration.

引用

页码：15186 / 15194

页数：9

共 42 条

[21] Photochemical Mechanisms of Fluorophores Employed in Single-Molecule Localization Microscopy [J].