From Microalgae to Biofuels: Investigating Valorization Pathways Towards Biorefinery Integration

The rapid growth of the world population led to an exponential growth in industrial activity all around the world. Consequently, CO2 emissions have risen almost 400% since 1950 due to human activities. In this context, microalgae biomass has emerged as a renewable and sustainable feedstock for producing third-generation biofuels. This study explores the laboratory-scale production of bioethanol and biomethane from dried algal biomass. The first step was to evaluate and optimize the production of glucose from the biomass. Thus, three different techniques with three different solvents were tested to identify the most effective and efficient in terms of saccharification yield. With the assistance of an autoclave or a high-temperature water bath and 0.2 M NaOH as a solvent, yields of 79.16 +/- 3.03% and 85.73 +/- 3.23% were achieved which correspond to 9.24 and 9.80 g/L of glucose, respectively. Furthermore, the most efficient method from the pretreatment step was chosen to carry out a factorial design to produce bioethanol. The experiments showed that the loading of cellulase was of crucial importance to the optimization of the process. Optimized ethanolic fermentation yielded ethanol concentrations up to 4.40 +/- 0.28 g/L (76.12 +/- 4.90%) (0.3 Mu NaOH, 750 mu L/gcellulose and 65 mu L/gstarch), demonstrating the critical role of cellulase loading. Biomethane potential (BMP) assays on fermentation residues showed increased yields compared to untreated feedstock, with a maximum methane yield of 217.88 +/- 10.40 mL/gVS. Combined energy production from bioethanol and biomethane was calculated at up to 1044.48 kWh/tn of algae feedstock, with biomethane contributing 75.26% to the total output. These findings highlight the potential of integrated algae-based biorefineries to provide scalable and sustainable biofuel solutions, aligning with circular economy principles.